Oertle M, Van Dyke R, Scharschmidt B F
Arch Int Physiol Biochim. 1984 Aug;92(2):107-18. doi: 10.3109/13813458409071134.
Very little is currently known about the ouabain-insensitive ATPase activity of the liver plasma membrane; we have therefore characterized it in plasma membranes from rat liver prepared using two different isolation techniques. Greater than 85% of ATPase activity in both preparations was ouabain-insensitive. Based on the effects of multiple inhibitors, including dicyclohexylcarbodiimide (DCCD), N-ethylmaleimide (NEM), vanadate, and oligomycin, the ouabain-insensitive ATPase activity of rat liver plasma membranes consists of a family of ATP-hydrolysing enzymes. Ouabain-insensitive ATPase activity was stimulated by HCO3-in both plasma membranes (13%) and mitochondria (69%) over the range of 7.5 to 9.0, and HCO3--stimulation was similarly inhibited by oligomycin in both preparations. A fraction of the ouabain-insensitive ATPase of liver plasma membranes is inhibited by DCCD and is resistent to inhibition by oligomycin; these characteristics are similar to those of the non-mitochondrial H+-ATPases recently described in lysosomes, endosomes, clathrin-coated vesicles, and Golgi from liver and other cell types.
目前对于肝细胞膜上哇巴因不敏感的ATP酶活性了解甚少；因此，我们使用两种不同的分离技术对大鼠肝脏制备的质膜中的该活性进行了表征。两种制备物中超过85%的ATP酶活性对哇巴因不敏感。基于多种抑制剂的作用，包括二环己基碳二亚胺（DCCD）、N-乙基马来酰亚胺（NEM）、钒酸盐和寡霉素，大鼠肝脏质膜中哇巴因不敏感的ATP酶活性由一系列ATP水解酶组成。在7.5至9.0的范围内，质膜（13%）和线粒体（69%）中的哇巴因不敏感ATP酶活性均受到HCO₃⁻的刺激，并且两种制备物中HCO₃⁻的刺激均同样受到寡霉素的抑制。肝细胞膜中一部分哇巴因不敏感的ATP酶受到DCCD的抑制，并且对寡霉素的抑制具有抗性；这些特征与最近在肝脏和其他细胞类型的溶酶体、内体、网格蛋白包被小泡和高尔基体中描述的非线粒体H⁺-ATP酶的特征相似。
