Mortensen R M, Rachubinski R A, Fujiki Y, Lazarow P B
Biochem J. 1984 Oct 15;223(2):547-50. doi: 10.1042/bj2230547.
Total RNA, extracted with guanidinium thiocyanate from liver of clofibrate-treated rats, was translated in vitro. Heating the RNA at 60 degrees C for 5 min before translation increased the synthesis of three peroxisomal polypeptides 10-100-fold. Preproalbumin synthesis increased 10-fold. Total incorporation of [35S]methionine into proteins merely doubled. Heating is essential for reproducible and adequate translation of mRNAs coding for peroxisomal and some other proteins.
用硫氰酸胍从氯贝丁酯处理的大鼠肝脏中提取的总RNA进行了体外翻译。在翻译前将RNA在60℃加热5分钟，可使三种过氧化物酶体多肽的合成增加10至100倍。前清蛋白的合成增加了10倍。[35S]甲硫氨酸掺入蛋白质的总量仅增加了一倍。加热对于编码过氧化物酶体和其他一些蛋白质的mRNA的可重复且充分的翻译至关重要。
