Sjödin L
Biochem Biophys Res Commun. 1984 Oct 30;124(2):578-84. doi: 10.1016/0006-291x(84)91593-6.
Binding of 125I-labelled Bolton-Hunter substance P (125I-BH-SP) to dispersed pancreatic acinar cells from guinea pigs was studied. Association of 125I-BH-SP to acinar cells was rapid, specific, and temperature-dependent. Dissociation of bound 125I-BH-SP was slow and 60 min after dilution only 12% of cell-associated radioactivity had dissociated from cells. Various c-terminal fragments of SP as well as structurally related substances inhibited binding. Bound 125I-BH-SP partly resisted acid washes of cells. After lysis of cells, cell-associated radioactivity was chromatographed on a Sephadex G-25 column. Part of radioactivity was eluted as apparently intact 125I-BH-SP. It is suggested that this material has been internalized into acinar cells.
研究了¹²⁵I标记的博尔顿-亨特物质P（¹²⁵I-BH-SP）与豚鼠分散胰腺腺泡细胞的结合情况。¹²⁵I-BH-SP与腺泡细胞的结合迅速、具有特异性且依赖温度。结合的¹²⁵I-BH-SP的解离缓慢，稀释60分钟后，仅12%与细胞相关的放射性从细胞中解离。SP的各种C末端片段以及结构相关物质抑制结合。结合的¹²⁵I-BH-SP部分抵抗细胞的酸洗。细胞裂解后，将与细胞相关的放射性在葡聚糖凝胶G-25柱上进行层析。部分放射性以明显完整的¹²⁵I-BH-SP形式洗脱。提示该物质已被内化到腺泡细胞中。
