人白血病K562细胞：5-氮杂胞苷对ε-、γ-珠蛋白和7SL RNA基因DNA甲基化的不同影响。
Human leukemic K562 cells: differential effects of 5-azacytidine on DNA methylation of epsilon-, gamma-globin and 7SL RNA genes.
作者信息
del Senno L, Conconi F, Barbieri R, Amelotti F, Bernardi F, Piva R, Viola L, Gambari R
出版信息
Boll Soc Ital Biol Sper. 1984 Aug 31;60(8):1613-9.
5 Azacytidine ribonucleoside (5 Aza CR), greatly enhances erythroid differentiation of the K562(h) cell line, with a sharp increase of embryonic and fetal globin gene expression. This phenomenon is correlated with the undermethylation of gamma-globin but not of epsilon-globin, as the epsilon-globin gene is already extensively undermethylated before 5AzaCR induction. By contrast no variations in both DNA methylation and expression are observed in 7SL RNA genes.
5-氮杂胞苷核糖核苷（5 Aza CR）极大地增强了K562(h)细胞系的红系分化，同时胚胎和胎儿珠蛋白基因表达急剧增加。这种现象与γ-珠蛋白的低甲基化相关，但与ε-珠蛋白无关，因为ε-珠蛋白基因在5AzaCR诱导之前就已经广泛低甲基化。相比之下，在7SL RNA基因中未观察到DNA甲基化和表达的变化。
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