对31种针对人IgG表位的小鼠单克隆抗体的评估。
Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes.
作者信息
Reimer C B, Phillips D J, Aloisio C H, Moore D D, Galland G G, Wells T W, Black C M, McDougal J S
出版信息
Hybridoma. 1984 Fall;3(3):263-75. doi: 10.1089/hyb.1984.3.263.
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2 anti-IgG3 hinge, 3 anti-IgG4 Fc, 3 anti-IgG4 Fd, 2 anti-nG4m(b), 4 anti-IgGFc, 2 anti-IgGFd, 1 anti-kappa, 1 anti-lambda, 1 anti-non IgG1, 2 anti-non IgG2, 2 anti-non-IgG3, 2 anti-non-IgG4. Evidence is presented validating their specificity. Some MAbs demonstrated to be avid, potent, and specific for well defined IgG-subclass epitopes may be partially or completely inactive in other assay systems, presumably because of different presentations of antigen epitopes. In general, this problem requires careful writing of protocols describing the use of MAbs.
获得了31个产生抗人IgG抗原决定簇单克隆抗体（MAb）的小鼠杂交瘤稳定克隆。所描述的具有不同特异性的杂交瘤数量如下：2个抗IgG1 Fc、1个抗IgG2 Fc、1个抗IgG2 Fd、2个抗IgG3 Fc、2个抗IgG3铰链区、3个抗IgG4 Fc、3个抗IgG4 Fd、2个抗nG4m(b)、4个抗IgGFc、2个抗IgGFd、1个抗κ、1个抗λ、1个抗非IgG1、2个抗非IgG2、2个抗非IgG3、2个抗非IgG4。文中提供了验证其特异性的证据。一些对明确的IgG亚类表位具有亲和力、效力强且特异性高的单克隆抗体，在其他检测系统中可能部分或完全无活性，推测是由于抗原表位的呈现方式不同。一般来说，这个问题需要仔细编写描述单克隆抗体使用的方案。
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