Nadelhaft I
J Histochem Cytochem. 1984 Dec;32(12):1344-6. doi: 10.1177/32.12.6209331.
A novel method for the immunohistochemical processing of free-floating tissue sections is described. Sections are immersed within drops of solution arranged on a hydrophobic surface. The procedure consists of sequentially suctioning away one fluid drop and replacing it by another, while the section remains in place. The technique permits easy testing of different antiserum dilutions, comparisons among different immunohistochemical protocols, and comparison of different antisera on serial tissue sections. Comparison is made to processing mounted sections.
描述了一种用于游离组织切片免疫组织化学处理的新方法。将切片浸入排列在疏水表面上的液滴中。该过程包括依次吸去一个液滴并用另一个液滴替换，而切片保持在原位。该技术允许轻松测试不同的抗血清稀释度，比较不同的免疫组织化学方案，以及在连续组织切片上比较不同的抗血清。并与处理裱贴切片进行了比较。
