Sbaschnig-Agler M, Ledeen R W, Grafstein B, Alpert R M
J Neurosci Res. 1984;12(2-3):221-32. doi: 10.1002/jnr.490120210.
Axonally transported radioactivity in sialoglycoconjugates, labeled by intraocular injection of [3H]N-acetylmannosamine, increased significantly during regeneration of goldfish optic axons at 30 degrees C. Ganglioside radioactivity showed the largest increase--approximately eightfold--in the optic nerve tract at 8 days after optic nerve crush while sialoglycoprotein radioactivity increased fourfold under the same conditions. As regeneration proceeded the magnitude of the increase in the nerve tract diminished for both glycoconjugates. In the optic tectum, however, transported radioactivities remained approximately twofold higher than controls between 15 and 25 days postcrush. The zwitterionic fraction of glycerophospholipids, labeled by intraocular injection of [14C]glycerol, also showed large increases during regeneration, but the acidic glycerophospholipids showed only modest increases. Thus while membrane components in general were elevated during the early stages of regeneration, the most pronounced increases occurred in gangliosides and certain glycerophospholipids. The significance of these changes in the regeneration process remain to be determined.
通过眼内注射[3H] - N - 乙酰甘露糖胺标记的唾液酸糖缀合物中的轴突运输放射性，在30℃下金鱼视神经轴突再生期间显著增加。在视神经挤压后8天，神经节苷脂放射性增加最大——约八倍——而在相同条件下唾液酸糖蛋白放射性增加四倍。随着再生的进行，两种糖缀合物在神经束中的增加幅度均减小。然而，在视顶盖中，挤压后15至25天内运输的放射性仍比对照高约两倍。通过眼内注射[14C]甘油标记的甘油磷脂两性离子部分在再生期间也显示出大幅增加，但酸性甘油磷脂仅显示适度增加。因此，虽然在再生早期一般膜成分会升高，但最显著的增加发生在神经节苷脂和某些甘油磷脂中。这些变化在再生过程中的意义仍有待确定。
