Apple R, Knauper B, Pesce A, Michael G
Mol Immunol. 1984 Oct;21(10):901-5. doi: 10.1016/0161-5890(84)90146-9.
The antigenic relationship between native and denatured bovine serum albumin (BSA) was investigated by examining specificity of antibodies directed against both proteins. Polyclonal antibodies produced to native and denatured BSA in hyperimmunized mice were specific for the homologous molecule. In contrast, polyclonal antibodies formed early in the response to native BSA reacted both the denatured and the native molecule. Monoclonal antibodies to native BSA were specific for the homologous antigen, while one of three clones prepared by immunization with denatured BSA produced an antibody cross-reactive with native BSA. Our results suggest that shared determinants of native and denatured BSA molecules can be recognized by both B- and T-cells.
通过检测针对天然和变性牛血清白蛋白（BSA）的抗体特异性，研究了天然和变性牛血清白蛋白之间的抗原关系。在超免疫小鼠中产生的针对天然和变性BSA的多克隆抗体对同源分子具有特异性。相比之下，在对天然BSA的反应早期形成的多克隆抗体与变性和天然分子都发生反应。针对天然BSA的单克隆抗体对同源抗原具有特异性，而通过用变性BSA免疫制备的三个克隆中的一个产生了与天然BSA交叉反应的抗体。我们的结果表明，天然和变性BSA分子的共同决定簇可被B细胞和T细胞识别。
