伴刀豆球蛋白A处理的淋巴细胞的苏氨酸饥饿会损害DNA聚合酶α的活性。

Threonine starvation of concanavalin A treated lymphocytes impairs DNA polymerase alpha activity.

作者信息

Taudou G, Wiart J, Panijel J

出版信息

Mol Immunol. 1984 Oct;21(10):949-53. doi: 10.1016/0161-5890(84)90152-4.

PMID:6209565

Abstract

When cultured in a threonine-deficient medium, concanavalin A treated guinea-pig lymphocytes do not incorporate tritiated thymidine. DNA polymerase activity is strongly affected. The addition of the missing amino acid is followed by an early increase in protein and RNA synthesis and a delayed rise in DNA polymerase alpha activity associated with the onset of DNA synthesis.

摘要

在缺乏苏氨酸的培养基中培养时，伴刀豆球蛋白A处理的豚鼠淋巴细胞不掺入氚标记的胸腺嘧啶核苷。DNA聚合酶活性受到强烈影响。添加缺失的氨基酸后，蛋白质和RNA合成早期增加，与DNA合成开始相关的DNA聚合酶α活性延迟上升。