[用氯化铬制备用于被动血凝试验的结合物的试验及其在口蹄疫病毒研究中的应用]

[Trials to obtain conjugates for the passive hemagglutination test with chromium chloride and their use in studying foot-and-mouth disease viruses].

作者信息

Tekerlekov P, Nikolova E

出版信息

Vet Med Nauki. 1984;21(7-8):15-21.

PMID:6209849

Abstract

Experiments were carried out to produce conjugates for the hemagglutination-inhibition reaction with the aid of chromic chloride. Studies with reference F.M.D. viruses and with CFT revealed that the antibody erythrocyte diagnostic agents were highly specific, and were stable in storage at +4 degrees C and in freeze-drying. It was found that the conjugates obtained with F.M.D. antigens possessed high specificity, however, were not stable and 7 to 10 days later lost their activity. Tests with the hemagglutination-reaction showed that immunity itself could be studied in cattle treated with F.M.D. vaccines. A correlation was found between the results obtained via the hemagglutination reaction and those obtained with the aid of the virus-neutralization test.

摘要

开展了借助氯化铬制备用于血凝抑制反应的结合物的实验。对口蹄疫病毒及补体结合试验的研究表明，抗体红细胞诊断试剂具有高度特异性，在4℃储存及冻干状态下均稳定。发现用口蹄疫抗原获得的结合物具有高度特异性，但不稳定，7至10天后会失去活性。血凝反应测试表明，在用口蹄疫疫苗处理过的牛中可以研究其自身免疫力。发现通过血凝反应获得的结果与借助病毒中和试验获得的结果之间存在相关性。