Tokuda G, Warrington R E
Appl Microbiol. 1970 Jul;20(1):35-9. doi: 10.1128/am.20.1.35-39.1970.
A passive hemagglutination test has been developed to detect and measure foot-and-mouth disease virus (FMDV) antibody by using glutaraldehyde as a coupling reagent. An optimal concentration of 10 to 40 mug of virus per ml with 0.25% glutaraldehyde at 25 C for 1 hr was established for the sensitization of sheep erythrocytes. A reaction time of 18 hr at 4 C or 2 hr at 37 C induced good agglutination in the presence of specific antibody. Sensitization was carried out in phosphate buffer, whereas agglutination and preadsorption of nonspecific agglutinins from sera were performed in gelatin (0.1%, w/v)-stabilized, phosphate-buffered saline. An optimal pH of 7.2 was also established for all reactions. Antibodies derived from guinea pigs hyperimmunized by infecting with FMDV, types A, O, and C were both virus-and type-specific. Preliminary experiments showed that strain A-119 and strain A-24 Cruzeiro could also be distinguished by hemagglutination. Parallel hemagglutination and complement-fixation tests showed the former to be two to four times more sensitive than the latter.
已开发出一种被动血凝试验，通过使用戊二醛作为偶联试剂来检测和测量口蹄疫病毒（FMDV）抗体。确定了在25℃下用0.25%戊二醛将每毫升10至40微克病毒致敏绵羊红细胞1小时的最佳浓度。在特异性抗体存在的情况下，4℃反应18小时或37℃反应2小时可诱导良好的凝集。致敏在磷酸盐缓冲液中进行，而血清中非特异性凝集素的凝集和预吸附在明胶（0.1%，w/v）稳定的磷酸盐缓冲盐水中进行。还确定了所有反应的最佳pH值为7.2。通过感染A、O和C型口蹄疫病毒超免疫的豚鼠产生的抗体具有病毒特异性和型特异性。初步实验表明，A-119株和A-24 Cruzeiro株也可通过血凝反应区分。平行血凝试验和补体结合试验表明，前者的敏感性比后者高两到四倍。
