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[使用荧光甲臜对单细胞氧化还原活性进行脉冲细胞荧光测定法]

[Impulse cytofluorometry of the redox activity of single cells using fluorescent formazan].

作者信息

Severin E, Stellmach J

出版信息

Acta Histochem. 1984;75(1):101-5.

PMID:6209908
Abstract

The first application of a newly developed fluorescent formazan in flow cytometry is described. The cell surface redox activity of isolated mouse hepatocytes after incubation with the tetrazolium salt forming the new fluorescent formazan and the DNA content after Hoechst staining have been measured simultaneously. 2 parametric distribution patterns have been obtained. This new and sensitive fluorometric technique can be used for automatic measurements of single cells correlating redox activity with other cell parameters.

摘要

本文描述了一种新开发的荧光甲臜在流式细胞术中的首次应用。在与形成新荧光甲臜的四氮唑盐孵育后,同时测量了分离的小鼠肝细胞的细胞表面氧化还原活性以及经Hoechst染色后的DNA含量。获得了两种参数分布模式。这种新型且灵敏的荧光测定技术可用于自动测量单个细胞,将氧化还原活性与其他细胞参数相关联。

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