用特异性cDNA评估糖皮质激素和环磷酸腺苷对大鼠肝脏氨甲酰磷酸合成酶mRNA水平的调控
Regulation of mRNA levels of rat liver carbamoylphosphate synthetase by glucocorticosteroids and cyclic AMP as estimated with a specific cDNA.
作者信息
de Groot C J, van Zonneveld A J, Mooren P G, Zonneveld D, van den Dool A, van den Bogaert A J, Lamers W H, Moorman A F, Charles R
出版信息
Biochem Biophys Res Commun. 1984 Nov 14;124(3):882-8. doi: 10.1016/0006-291x(84)91040-4.
The construction and cloning of a cDNA complementary to the mRNA of rat liver carbamoylphosphate synthetase (ammonia) is described. Using this cDNA, the size of the mature, cytosolic carbamoylphosphate synthetase (ammonia) mRNA is estimated to be 6.0 Kb. The levels of carbamoylphosphate synthetase (ammonia) mRNA in liver are shown to be regulated by glucocorticosteroids and cyclic AMP. By studying mRNA levels of carbamoylphosphate synthetase, albumin and phosphoenolpyruvate carboxykinase, using specific cDNA clones, we show that carbamoylphosphate synthetase gene expression, like that of albumin is liver-specific.
本文描述了与大鼠肝脏氨甲酰磷酸合成酶（氨）mRNA互补的cDNA的构建与克隆。利用该cDNA，估计成熟的胞质氨甲酰磷酸合成酶（氨）mRNA的大小为6.0 kb。结果表明，肝脏中氨甲酰磷酸合成酶（氨）mRNA的水平受糖皮质激素和环磷酸腺苷的调控。通过使用特异性cDNA克隆研究氨甲酰磷酸合成酶、白蛋白和磷酸烯醇式丙酮酸羧激酶的mRNA水平，我们发现氨甲酰磷酸合成酶基因的表达与白蛋白一样具有肝脏特异性。
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