Moorman A F, de Boer P A, Charles R, Lamers W H
Department of Anatomy and Embryology, University of Amsterdam, The Netherlands.
FEBS Lett. 1990 Dec 10;276(1-2):9-13. doi: 10.1016/0014-5793(90)80494-4.
A hybridocytochemical analysis of adult liver from normal control and from hormonally and dietary-treated rats was carried out, using radioactively-labelled probes for the mRNAs of glutamine synthetase (GS), carbamoylphosphate synthetase (CPS) and phosphoenolpyruvate carboxykinase (PEPCK). In line with previous findings, GS mRNA is exclusively expressed in a small pericentral compartment, CPS mRNA exclusively in a contiguous large periportal compartment and PEPCK mRNA across the entire porto-central distance. The density of labelling in CPS and PEPCK mRNA-positive hepatocytes decreases in a porto-central direction. Starvation resulted in a reversal of the gradient of CPS mRNA within its periportal compartment; glucose refeeding counteracted this effect. Livers of glucocorticosteroid-treated, starved or diabetic rats also revealed a reversal of the normal gradient of CPS mRNA, but now across the entire porto-central distance. The patterns of expression of GS and PEPCK mRNA remained essentially unchanged, notwithstanding substantial changes in the levels of expression. It is concluded that blood-borne factors constitute the major determinants for the expression patterns of CPS mRNA within the context of the architecture of the liver lobulus.
利用放射性标记的谷氨酰胺合成酶（GS）、氨基甲酰磷酸合成酶（CPS）和磷酸烯醇式丙酮酸羧激酶（PEPCK）mRNA探针，对正常对照以及经激素和饮食处理的成年大鼠肝脏进行了杂交细胞化学分析。与之前的研究结果一致，GS mRNA仅在中央静脉周围的一个小区域表达，CPS mRNA仅在相邻的门静脉周围大区域表达，而PEPCK mRNA在整个门静脉-中央静脉距离范围内均有表达。CPS和PEPCK mRNA阳性肝细胞中的标记密度沿门静脉-中央静脉方向降低。饥饿导致CPS mRNA在其门静脉周围区域内的梯度反转；重新喂食葡萄糖可抵消这种效应。经糖皮质激素处理、饥饿或患糖尿病大鼠的肝脏也显示出CPS mRNA正常梯度的反转，但现在是在整个门静脉-中央静脉距离范围内。尽管表达水平发生了显著变化，但GS和PEPCK mRNA的表达模式基本保持不变。得出的结论是，在肝小叶结构的背景下，血源性因素是CPS mRNA表达模式的主要决定因素。
