犬淋巴细胞亚群的血清学鉴定与分离
Serological identification and separation of canine lymphocyte subpopulations.
作者信息
Holler E, Kolb H J, Bodenberger U, Eckstein R, Hoffmann-Fezer G, Linke R P, Mempel W, Rodt H, Thierfelder S
出版信息
Exp Hematol. 1984 Nov;12(10):774-81.
In vitro treatment of bone marrow grafts with absorbed rabbit-antidog thymocyte globulin (ATG) prevented graft-versus-host disease in a substantial number of the dogs differing by one DLA haplotype. Absorbed ATG has now been used for serological identification of canine lymphocyte populations. Specific labeling of canine T-lymphocytes by absorbed ATG could be demonstrated by (a) a distribution of ATG-positive cells in suspensions of canine lymphoid organs similar to that of T cells observed in other species and by specific staining of paracortical thymus-dependent lymph node areas in immunohistochemistry, (b) complementary labeling of nylon-wool-separated cells by ATG and antiimmunoglobulin sera, and (c) correlation of ATG surface labeling with responder activities in mixed lymphocyte cultures.
用吸附的兔抗犬胸腺细胞球蛋白（ATG）对骨髓移植物进行体外处理，在大量仅相差一个犬白细胞抗原（DLA）单倍型的犬中预防了移植物抗宿主病。吸附的ATG现已用于犬淋巴细胞群体的血清学鉴定。吸附的ATG对犬T淋巴细胞的特异性标记可通过以下方式证明：（a）在犬淋巴器官悬液中，ATG阳性细胞的分布与在其他物种中观察到的T细胞分布相似，且在免疫组织化学中副皮质区胸腺依赖淋巴结区域有特异性染色；（b）ATG和抗免疫球蛋白血清对尼龙毛分离细胞的互补标记；（c）在混合淋巴细胞培养中，ATG表面标记与反应活性的相关性。
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