Langhorne J, Lindahl K F
Eur J Immunol. 1982 Feb;12(2):101-6. doi: 10.1002/eji.1830120202.
A limiting dilution assay was used to compare the frequency of cytotoxic T lymphocyte precursors (CTL-P) which respond to H-2 antigens presented with additional background differences, with the frequency of CTL-P which respond to H-2 antigens on a self background. Individual cultures were divided and assayed for cytotoxic activity on the two targets sharing H-2, but not the background; no cultures were seen which clearly killed one and not the other, and the same frequency of CTL-P was measured on target cells that differed from the responder only at H-2 and on target cells that differed also in the background, irrespective of the background of the stimulator. Thus, the assumption that allospecific cytotoxic T lymphocytes (CTL) recognize H-2 plus minor histocompatibility antigens dose not serve as an adequate explanation for the high frequency of allospecific CTL. The data also suggest that the two allelic forms of beta2-microglobulin do not contribute to the alloantigenic determinant.
采用有限稀释分析法比较了对具有额外背景差异的H-2抗原作出反应的细胞毒性T淋巴细胞前体（CTL-P）的频率，以及对自身背景上的H-2抗原作出反应的CTL-P的频率。将各个培养物分开，并针对两种共享H-2但背景不同的靶标检测其细胞毒性活性；未观察到能明确杀伤其中一个靶标而不杀伤另一个靶标的培养物，并且在仅在H-2上与应答者不同的靶细胞以及在背景上也不同的靶细胞上测量到相同频率的CTL-P，而与刺激物的背景无关。因此，关于同种特异性细胞毒性T淋巴细胞（CTL）识别H-2加次要组织相容性抗原的假设并不能充分解释同种特异性CTL的高频率。数据还表明，β2-微球蛋白的两种等位基因形式对同种抗原决定簇没有贡献。
