痘病毒诱导的及病毒粒子所含脱氧核糖核酸酶在DNA聚丙烯酰胺凝胶中的原位检测
In situ assay of poxvirus-induced and virion-contained deoxyribonucleases in DNA polyacrylamide gels.
作者信息
Zöllner E J, Zahn R K, Jungwirth C
出版信息
Intervirology. 1978;9(1):1-7. doi: 10.1159/000148915.
PMID:621135
Abstract
Cowpox virus and vaccinia virus WR specific DNases were identified by an in situ assay using DNA-containing acrylamide gels. A DNase present in the isolated virions of strain cowpox and vaccinia WR was identified. Its properties as determined by the in situ assay resembled those of the well-characterized DNase V1. The assay was also used to follow the time course of 'acid' DNase induction by cowpox virus in primary chick embryo fibroblasts.
摘要
通过使用含DNA的丙烯酰胺凝胶的原位测定法鉴定了牛痘病毒和痘苗病毒WR特异性脱氧核糖核酸酶。在分离出的牛痘病毒株和痘苗病毒WR的病毒粒子中鉴定出一种脱氧核糖核酸酶。通过原位测定法确定的其特性与特征明确的脱氧核糖核酸酶V1的特性相似。该测定法还用于追踪牛痘病毒在原代鸡胚成纤维细胞中诱导“酸性”脱氧核糖核酸酶的时间进程。
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