Hang K F, Anastassiadis P A
Poult Sci. 1982 Jan;61(1):156-60. doi: 10.3382/ps.0610156.
The incorporation of D-[1-14C]-labeled glucosamine (GlcN) and D-[1-14C]-labeled galactosamine (GalN) into mucopolysaccharide-peptide complex(es) (MPS-P) and the rate of 14CO2 production by tissue slices of skin, comb, liver, kidney, shell gland, and magnum from laying hens were studied during a 12 hr period. The D-[1-14C] GlcN was metabolized at a faster rate than D-[1-14C] GalN. No 14CO2 was produced by skin and comb tissues incubated with D-[1-14C]GalN for 12 hr. The amount of 14C associated with the acetone extract of the tissues, acetone-extracted tissues, and MPS-P of the tissue increased with increasing incubation time, but generally the increase was highest in the MPS-P. A comparison among the tissues indicated that the radioactivity present in CO2 and MPS-P was highest in the shell gland and lowest in comb tissue slices. The rates of incorporation of 14C-hexosamine (HexN) into MPS-P by tissue slices appeared to be in general agreement to those of intact animals.
在12小时期间，研究了将D-[1-14C]标记的葡萄糖胺（GlcN）和D-[1-14C]标记的半乳糖胺（GalN）掺入黏多糖-肽复合物（MPS-P）的情况，以及蛋鸡皮肤、鸡冠、肝脏、肾脏、壳腺和输卵管膨大部组织切片产生14CO2的速率。D-[1-14C] GlcN的代谢速率比D-[1-14C] GalN快。用D-[1-14C]GalN孵育12小时的皮肤和鸡冠组织未产生14CO2。与组织的丙酮提取物、丙酮提取组织和组织的MPS-P相关的14C量随孵育时间的增加而增加，但一般在MPS-P中增加最高。组织间的比较表明，CO2和MPS-P中的放射性在壳腺中最高，在鸡冠组织切片中最低。组织切片将14C-己糖胺（HexN）掺入MPS-P的速率似乎与完整动物的情况总体一致。
