Dibák O, Krajcovicová M, Kopec Z
Physiol Bohemoslov. 1982;31(2):183-92.
Using diets with a mounting egg albumin and mutton protein concentration, the authors determined the optimum physiological doses, which are identical with the maximum of linearity of the given parameters, from changes in body nitrogen, body weight and body water. The regression equations of the lines from these parameters were used to determine the minimum physiological doses for newly weaned rats. The optimum and minimum physiological doses of the amino acids in the relevant dietary proteins were also determined by analysing the source for its nitrogen content and other components and for the amino acid spectrum. The optimum dose of egg albumin protein for newly weaned rats was 1.69 g/day (on a 15% protein diet) and of mutton protein 2.56 g/day (on a 12.5% protein diet), with daily amounts of 60 mg methionine, 93 mg phenylalanine, 112 mg valine and 60 mg tyrosine for both proteins. The minimum doses of egg albumin and mutton protein, determined from changes in body nitrogen were 282 mg and 213 mg/day respectively [methionine 10.4 (4.7) mg/day, phenylalanine 16.1 (7.5) mg/day, valine 19.1 (9.2) mg/day and tyrosine 9.8 (5.2) mg/day)].
作者通过使用蛋清蛋白和羊肉蛋白浓度不断增加的日粮，根据体氮、体重和体水的变化，确定了最佳生理剂量，该剂量与给定参数的最大线性度相同。利用这些参数的直线回归方程来确定刚断奶大鼠的最低生理剂量。还通过分析相关日粮蛋白质的氮含量、其他成分以及氨基酸谱，确定了其中氨基酸的最佳和最低生理剂量。刚断奶大鼠的蛋清蛋白最佳剂量为1.69克/天（在15%蛋白质日粮中），羊肉蛋白最佳剂量为2.56克/天（在12.5%蛋白质日粮中），两种蛋白质的蛋氨酸、苯丙氨酸、缬氨酸和酪氨酸每日含量分别为60毫克、93毫克、112毫克和60毫克。根据体氮变化确定的蛋清蛋白和羊肉蛋白最低剂量分别为282毫克/天和213毫克/天[蛋氨酸10.4（4.7）毫克/天，苯丙氨酸16.1（7.5）毫克/天，缬氨酸19.1（9.2）毫克/天，酪氨酸9.8（5.2）毫克/天]。
