Quantitation of proteins by densitometric scanning of immunoelectrophoretic precipitates.

Densitometric scanning of stained immunoelectrophoretic precipitates of nine human serum proteins showed that the optical density of the precipitates is approximately proportional to the molar concentration of antigen in the sample. Deviations from this correlation were noted for the macroglobulins (mol. wt. greater than 15 x 10(4)). The resolving capacity of the line immunoelectrophoretic procedure permits densitometric valuations of composite patterns.