Biral D, Damiani E, Volpe P, Salviati G, Margreth A
Biochem J. 1982 Jun 1;203(3):529-40. doi: 10.1042/bj2030529.
Antibodies specific for rabbit fast-twitch-muscle myosin LCIF light chain were purified by affinity chromatography and characterized by both non-competitive and competitive enzyme-linked immunosorbent assay (ELISA) and a gel-electrophoresis-derived assay (GEDELISA). The antibodies did not cross-react with myosin heavy chains, and were weakly cross-reactive with the LC2F [5,5'-dithio-(2-nitrobenzoic acid)-dissociated] light chain and with all classes of dissociated light chains (LC1Sa, LC1Sb and LC2S), as well as with the whole myosin, from hind-limb slow-twitch muscle. The immunoreactivity of myosins with a truly mixed light-chain pattern (e.g. vastus lateralis and gastrocnemius) correlated with percentage content of fast-twitch-muscle-type light chains. A more extensive immunoreactivity was observed with diaphragm and masseter myosins, which were also characterized, respectively, by a relative or absolute deficiency of LC1Sa light chain. Furthermore, it was found that the LC1Sb light chain of masseter myosin is antigenically different from its slow-twitch-muscle myosin analogue, and is immunologically related to the LC1F light chain. Rabbit masseter muscle from its metabolic and physiological properties and the content, activity and immunological properties of sarcoplasmic-reticulum adenosine triphosphatase, is classified as a red, predominantly fast-twitch, muscle. Therefore our results suggest that the two antigenically different iso-forms of LC1Sb light chain are associated with the myosins of fast-twitch red and slow-twitch red fibres respectively.
通过亲和层析法纯化了针对兔快肌肌球蛋白轻链LC1F的特异性抗体，并通过非竞争性和竞争性酶联免疫吸附测定（ELISA）以及凝胶电泳衍生测定（GEDELISA）对其进行了表征。这些抗体与肌球蛋白重链无交叉反应，与LC2F [5,5'-二硫代-(2-硝基苯甲酸)-解离]轻链以及后肢慢肌所有解离轻链类别（LC1Sa、LC1Sb和LC2S）以及整个肌球蛋白有弱交叉反应。具有真正混合轻链模式的肌球蛋白（如股外侧肌和腓肠肌）的免疫反应性与快肌型轻链的百分比含量相关。在膈肌和咬肌肌球蛋白中观察到更广泛的免疫反应性，它们还分别以LC1Sa轻链相对或绝对缺乏为特征。此外，发现咬肌肌球蛋白的LC1Sb轻链与其慢肌肌球蛋白类似物在抗原性上不同，并且在免疫上与LC1F轻链相关。兔咬肌从其代谢和生理特性以及肌浆网三磷酸腺苷酶的含量、活性和免疫特性来看，被归类为红色、主要为快肌的肌肉。因此，我们的结果表明，两种抗原性不同的LC1Sb轻链同工型分别与快肌红色纤维和慢肌红色纤维的肌球蛋白相关。
