• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli.从粗糙脉孢菌和大肠杆菌中纯化得到的粗糙脉孢菌分解代谢脱氢奎尼酸酶的特性分析。
Biochem J. 1982 Jun 1;203(3):769-73. doi: 10.1042/bj2030769.
2
Expression in Escherichia coli K-12 of the structural gene for catabolic dehydroquinase of Neurospora crassa.粗糙脉孢菌分解代谢脱氢奎尼酸酶结构基因在大肠杆菌K-12中的表达。
Proc Natl Acad Sci U S A. 1977 Aug;74(8):3508-12. doi: 10.1073/pnas.74.8.3508.
3
Purification and characterization of 3-dehydroquinase from Escherichia coli.大肠杆菌3-脱氢奎尼酸酶的纯化与特性分析
Biochem J. 1986 Nov 1;239(3):699-704. doi: 10.1042/bj2390699.
4
Characterization of the 3-dehydroquinase domain of the pentafunctional AROM protein, and the quinate dehydrogenase from Aspergillus nidulans, and the overproduction of the type II 3-dehydroquinase from neurospora crassa.构巢曲霉五功能AROM蛋白的3-脱氢奎尼酸酶结构域、奎尼酸脱氢酶的特性，以及粗糙脉孢菌II型3-脱氢奎尼酸酶的过量表达
Biochem J. 1993 Dec 1;296 ( Pt 2)(Pt 2):451-7. doi: 10.1042/bj2960451.
5
Purification and characterization of catabolic dehydroquinase, an enzyme in the inducible quinic acid catabolic pathway of Neurospora crassa.分解代谢脱氢奎尼酸酶的纯化与特性分析，该酶存在于粗糙脉孢菌可诱导的奎尼酸分解代谢途径中。
J Biol Chem. 1975 Aug 10;250(15):6008-14.
6
Effect of mutations in the qa gene cluster of Neurospora crassa on the enzyme catabolic dehydroquinase.粗糙脉孢菌qa基因簇突变对酶促分解脱氢奎尼酸酶的影响。
J Bacteriol. 1975 Oct;124(1):491-6. doi: 10.1128/jb.124.1.491-496.1975.
7
Constitutive expression in Escherichia coli of the Neurospora crassa structural gene encoding the inducible enzyme catabolic dehydroquinase.编码诱导型酶分解代谢脱氢奎尼酸酶的粗糙脉孢菌结构基因在大肠杆菌中的组成型表达。
Mol Gen Genet. 1979 Apr 17;172(1):93-8. doi: 10.1007/BF00276219.
8
Transcription and translation in E. coli of hybrid plasmids containing the catabolic dehydroquinase gene from Neurospora crassa.含有来自粗糙脉孢菌的分解代谢脱氢奎尼酸酶基因的杂交质粒在大肠杆菌中的转录和翻译。
Gene. 1978 Nov;4(3):241-59. doi: 10.1016/0378-1119(78)90021-5.
9
The overexpression, purification and complete amino acid sequence of chorismate synthase from Escherichia coli K12 and its comparison with the enzyme from Neurospora crassa.大肠杆菌K12分支酸合酶的过表达、纯化及完整氨基酸序列，及其与粗糙脉孢菌该酶的比较。
Biochem J. 1988 Apr 15;251(2):313-22. doi: 10.1042/bj2510313.
10
Expression of the structural gene for catabolic dehydroquinase of Neurospora crassa in Escherichia coli K12.粗糙脉孢菌分解代谢脱氢奎尼酸酶结构基因在大肠杆菌K12中的表达。
Brookhaven Symp Biol. 1977(29):297-308.

引用本文的文献

1
Characterization of the 3-dehydroquinase domain of the pentafunctional AROM protein, and the quinate dehydrogenase from Aspergillus nidulans, and the overproduction of the type II 3-dehydroquinase from neurospora crassa.构巢曲霉五功能AROM蛋白的3-脱氢奎尼酸酶结构域、奎尼酸脱氢酶的特性，以及粗糙脉孢菌II型3-脱氢奎尼酸酶的过量表达
Biochem J. 1993 Dec 1;296 ( Pt 2)(Pt 2):451-7. doi: 10.1042/bj2960451.
2
Cloning and characterization of the three enzyme structural genes QUTB, QUTC and QUTE from the quinic acid utilization gene cluster in Aspergillus nidulans.构巢曲霉中奎尼酸利用基因簇的三个酶结构基因QUTB、QUTC和QUTE的克隆与特性分析
Curr Genet. 1985;9(4):305-11. doi: 10.1007/BF00419960.
3
The purification of shikimate dehydrogenase from Escherichia coli.从大肠杆菌中纯化莽草酸脱氢酶。
Biochem J. 1985 Feb 15;226(1):217-23. doi: 10.1042/bj2260217.
4
Sequencing and overexpression of the Escherichia coli aroE gene encoding shikimate dehydrogenase.编码莽草酸脱氢酶的大肠杆菌aroE基因的测序与过表达。
Biochem J. 1988 Jan 15;249(2):319-26. doi: 10.1042/bj2490319.
5
Sequence analysis and transformation by the catabolic 3-dehydroquinase (QUTE) gene from Aspergillus nidulans.来自构巢曲霉的分解代谢3-脱氢奎尼酸酶（QUTE）基因的序列分析与转化
Biochem J. 1986 Dec 1;240(2):481-8. doi: 10.1042/bj2400481.
6
Gene organization and regulation in the qa (quinic acid) gene cluster of Neurospora crassa.粗糙脉孢菌喹啉酸（qa）基因簇中的基因组织与调控
Microbiol Rev. 1985 Sep;49(3):338-58. doi: 10.1128/mr.49.3.338-358.1985.
7
The Mycobacterium tuberculosis shikimate pathway genes: evolutionary relationship between biosynthetic and catabolic 3-dehydroquinases.结核分枝杆菌莽草酸途径基因：生物合成和分解代谢3-脱氢奎尼酸酶之间的进化关系。
Mol Gen Genet. 1991 Sep;228(3):385-92. doi: 10.1007/BF00260631.

本文引用的文献

1
DISC ELECTROPHORESIS. II. METHOD AND APPLICATION TO HUMAN SERUM PROTEINS.圆盘电泳。II. 方法及其在人血清蛋白中的应用。
Ann N Y Acad Sci. 1964 Dec 28;121:404-27. doi: 10.1111/j.1749-6632.1964.tb14213.x.
2
The involvement of proteolysis in conformational stability of the carbamoyl-phosphate synthetase/aspartate carbamoyltransferase enzyme of Neurospora crassa.蛋白酶解参与粗糙脉孢菌氨甲酰磷酸合成酶/天冬氨酸氨甲酰转移酶的构象稳定性。
Biochim Biophys Acta. 1981 Oct 13;661(2):315-22. doi: 10.1016/0005-2744(81)90020-6.
3
High recovery of tryptophan from acid hydrolysates of proteins.从蛋白质酸水解物中高效回收色氨酸。
Biochem Biophys Res Commun. 1969 Apr 29;35(2):175-81. doi: 10.1016/0006-291x(69)90263-0.
4
Genetic location of certain mutations conferring recombination deficiency in Escherichia coli.大肠杆菌中某些导致重组缺陷的突变的基因定位。
J Bacteriol. 1969 Jan;97(1):244-9. doi: 10.1128/jb.97.1.244-249.1969.
5
The inducible quinate-shikimate catabolic pathway in Neurospora crassa: induction and regulation of enzyme synthesis.粗糙脉孢菌中可诱导的奎尼酸-莽草酸分解代谢途径：酶合成的诱导与调控
J Gen Microbiol. 1974 Apr;81(2):357-72. doi: 10.1099/00221287-81-2-357.
6
The inducible quinate-shikimate catabolic pathway in Neurospora crassa: genetic organization.粗糙脉孢菌中可诱导的奎尼酸-莽草酸分解代谢途径：基因组织
J Gen Microbiol. 1974 Apr;81(2):337-55. doi: 10.1099/00221287-81-2-337.
7
Studies on the apparent instability of Neurospora tryptophan synthase. Evidence for protease.关于粗糙脉孢菌色氨酸合酶明显不稳定性的研究。蛋白酶的证据。
Eur J Biochem. 1973 Jan 3;32(1):129-35. doi: 10.1111/j.1432-1033.1973.tb02588.x.
8
Coupling techniques in solid-phase sequencing.固相测序中的偶联技术。
Methods Enzymol. 1977;47:277-88. doi: 10.1016/0076-6879(77)47032-0.
9
New supports in solid-phase sequencing.固相测序中的新支持技术。
Methods Enzymol. 1977;47:263-77. doi: 10.1016/0076-6879(77)47031-9.
10
Intramolecular ionic interactions of lysine residues and a possible folding domain in fructose diphosphate aldolase.果糖二磷酸醛缩酶中赖氨酸残基的分子内离子相互作用及一个可能的折叠结构域
Biochem J. 1977 Jan 1;161(1):63-71. doi: 10.1042/bj1610063.

从粗糙脉孢菌和大肠杆菌中纯化得到的粗糙脉孢菌分解代谢脱氢奎尼酸酶的特性分析。

Characterization of Neurospora crassa catabolic dehydroquinase purified from N. crassa and Escherichia coli.

作者信息

Hawkins A R, Reinert W R, Giles N H

出版信息

Biochem J. 1982 Jun 1;203(3):769-73. doi: 10.1042/bj2030769.

DOI:10.1042/bj2030769
PMID:6214255
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1158294/
Abstract
  1. Neurospora crassa catabolic dehydroquinase has been purified from N. crassa and Escherichia coli. 2. Protein-sequence and gel-electrophoretic data show that apparently pure, homogeneous native dehydroquinase is a mixture of intact and proteinase-cleaved enzyme monomers. 3. Protein-sequence data and steady-state kinetics show that the catabolic dehydroquinase gene of N. crassa is expressed with fidelity in E. coli.
摘要
  1. 粗糙脉孢菌分解代谢脱氢奎尼酸酶已从粗糙脉孢菌和大肠杆菌中纯化出来。2. 蛋白质序列和凝胶电泳数据表明，看似纯净、均一的天然脱氢奎尼酸酶是完整酶单体和蛋白酶切割后的酶单体的混合物。3. 蛋白质序列数据和稳态动力学表明，粗糙脉孢菌的分解代谢脱氢奎尼酸酶基因在大肠杆菌中能准确表达。