Palladina T A, Petrova N V
Biokhimiia. 1982 Jul;47(7):1154-8.
The effects of the SH-groups binding agent p-chloromercurybenzoate (rho CMB) and the SH-containing compounds dithiothreitol (DTT), beta-mercaptoethanol (ME) and reduced glutathione (GSH) on activation by Mg2+ and K+ of ATPase in plasma membrane preparations from corn sprout root cells were studied. Rho CMB inhibited the ATPase activity, the degree of inhibition being directly dependent on the increase of the inhibitor concentration (from 10(-6) up to 10(-4) M); the inhibition was eliminated by the SH-containing agents (25 mM). DTT and ME added to the homogenization medium and ME added to the reaction mixture produced different effects on the ATPase activity of the membranes depending on the nature of the cations added. In the absence of the additives the ATPase activity was somewhat decreased, showing a sharp rise in the presence of Mg2+; an addition of K+ to a Mg2+-containing medium further increased the enzyme activity. GSH had no effect on the ATPase activation by the cations.
研究了巯基结合剂对氯汞苯甲酸（ρCMB）以及含巯基化合物二硫苏糖醇（DTT）、β-巯基乙醇（ME）和还原型谷胱甘肽（GSH）对玉米芽根细胞质膜制剂中Mg2+和K+激活ATP酶的影响。ρCMB抑制ATP酶活性，抑制程度直接取决于抑制剂浓度的增加（从10^(-6)到10^(-4)M）；含巯基试剂（25mM）可消除这种抑制作用。添加到匀浆介质中的DTT和ME以及添加到反应混合物中的ME，根据所添加阳离子的性质，对膜的ATP酶活性产生不同影响。在没有添加剂的情况下，ATP酶活性有所下降，在Mg2+存在时急剧上升；向含Mg2+的介质中添加K+进一步增加了酶活性。GSH对阳离子激活ATP酶没有影响。
