Fransson L A, Sjöberg I
Carbohydr Res. 1982 Jul 16;105(2):205-13. doi: 10.1016/s0008-6215(00)84968-x.
The ability of fibroblast heparan sulphate to aggregate was examined by affinity chromatography on agarose gels substituted with heparan sulphate variants from beef-lung tissue (designated HS2-A, HS3-A, and HS4-A) having different tendencies for association. Fibroblast heparan sulphate bound only to HS4-A agarose. The cell-derived material was subdivided into low-, medium-, and high-affinity species by affinity chromatography on HS4-A-agarose. High-affinity variants contained a larger proportion of chains of high molecular weight. Degradation of heparan sulphate by selective periodate-oxidation of glucuronate in regions rich in 2-acetamido-2-deoxyglucose followed by scission in alkali produced fragments comprising the highly irregular, iduronate-rich and N-sulphate-rich domains. The latter were slightly larger in the high-affinity form. By re-oxidation of the irregular domains, the location of glucuronate residues was identified. The degradation pattern, i.e., the distribution of iduronate-containing repeats, was markedly similar in the high-affinity variant and in the HS4-A chains that were used as affinity ligands. It is suggested that the strength and specificity of heparan sulphate self-association is dependent on complementary and co-operative associations between a number of contact zones.
通过在琼脂糖凝胶上进行亲和层析，利用来自牛肺组织的具有不同缔合倾向的硫酸乙酰肝素变体（分别命名为HS2 - A、HS3 - A和HS4 - A），研究了成纤维细胞硫酸乙酰肝素的聚集能力。成纤维细胞硫酸乙酰肝素仅与HS4 - A琼脂糖结合。通过在HS4 - A琼脂糖上进行亲和层析，将细胞衍生物质细分为低亲和力、中等亲和力和高亲和力的种类。高亲和力变体含有较大比例的高分子量链。通过在富含2 - 乙酰氨基 - 2 - 脱氧葡萄糖的区域对葡萄糖醛酸进行选择性高碘酸盐氧化，随后在碱中裂解，对硫酸乙酰肝素进行降解，产生了包含高度不规则、富含艾杜糖醛酸和富含N - 硫酸酯的结构域的片段。后者在高亲和力形式中略大。通过对不规则结构域进行再氧化，确定了葡萄糖醛酸残基的位置。高亲和力变体和用作亲和配体的HS4 - A链中的降解模式，即含艾杜糖醛酸重复序列的分布，明显相似。这表明硫酸乙酰肝素自缔合的强度和特异性取决于多个接触区域之间的互补和协同缔合。
