微量热法作为一种免疫学工具：用牛心线粒体三磷酸腺苷酶及其抗血清进行的初步研究。

Microcalorimetry as an immunological tool: preliminary studies with beef heart mitochondrial adenosine triphosphatase and its antiserum.

作者信息

Poore V M, Beezer A E

出版信息

J Immunol Methods. 1982 Aug 27;53(1):91-102. doi: 10.1016/0022-1759(82)90243-5.

Abstract

The calorimetric technique was applied to a complex immunological system based upon purified beef heart mitochondrial adenosine triphosphatase (ATPase). Whole serum containing antibodies to the ATPase was obtained from a rabbit and used to study the antigen-antiserum interaction in comparison with control serum pooled from 50 non-immune rabbits. The results suggest that the immunological interaction may be accompanied by complex processes which were not detectable by other immunological methods. It was shown that kinetic analysis of the calorimetric data may resolve an apparently complex process of interaction into different component stages of reaction. The advantages of this type of application of calorimetry are discussed.

摘要

量热技术被应用于一个基于纯化的牛心线粒体腺苷三磷酸酶（ATP酶）的复杂免疫系统。从一只兔子身上获取了含有针对该ATP酶抗体的全血清，并与从50只非免疫兔子中收集的对照血清一起用于研究抗原 - 抗血清相互作用。结果表明，免疫相互作用可能伴随着其他免疫方法无法检测到的复杂过程。结果显示，对量热数据的动力学分析可以将一个明显复杂的相互作用过程解析为不同的反应组分阶段。本文讨论了这种量热法应用类型的优点。