Benahmed M, Bernier M, Ducharme J R, Saez J M
Mol Cell Endocrinol. 1982 Nov-Dec;28(3):705-16. doi: 10.1016/0303-7207(82)90157-5.
Using a primary culture of purified immature pig Leydig cells we have demonstrated: (1) that during the first 3 days of culture there is a 'spontaneous' maturation of the steroidogenic response to hCG, as expressed by a 50-fold increase of the steroidogenic capacity, an increased secretion of both dehydroepiandrosterone sulfate (DHAS) and testosterone (T), a shift of the DHAS/T ratio (5 on day 0 vs. 0.5 on day 3) without significant changes in the number of hCG-binding sites; (2) that purified cells, on day 3 following hCG stimulation, secrete large amounts of T and small amounts of E2 (T/E2 congruent to 150) and detectable amounts of estrone, while crude pig interstitial cells under the same conditions secrete less T but 40-50 times more estrogens (T/estrogens congruent to 1.5); (3) that the steroidogenic responsiveness of purified Leydig cells is not impaired by E2 treatment, in spite of the fact that Leydig cells contain specific estradiol receptors (approximately 10000 sites/cell). These data suggest that in this model the main source of testicular estrogens are not Leydig cell but some other testicular cell types, and that the lack of effect of estrogens on pig Leydig cell steroidogenesis is not due to absence of estrogen receptors.
利用纯化的未成熟猪睾丸间质细胞原代培养物，我们已经证明：（1）在培养的前3天，对人绒毛膜促性腺激素（hCG）的类固醇生成反应存在“自发”成熟，表现为类固醇生成能力增加50倍，硫酸脱氢表雄酮（DHAS）和睾酮（T）的分泌增加，DHAS/T比值发生变化（第0天为5，第3天为0.5），而hCG结合位点数量无显著变化；（2）纯化细胞在hCG刺激后的第3天，分泌大量的T和少量的E2（T/E2约为150）以及可检测量的雌酮，而在相同条件下的粗制猪间质细胞分泌的T较少，但雌激素多40 - 50倍（T/雌激素约为1.5）；（3）尽管睾丸间质细胞含有特异性雌二醇受体（约10000个位点/细胞），但纯化的睾丸间质细胞的类固醇生成反应不受E2处理的影响。这些数据表明，在该模型中，睾丸雌激素的主要来源不是睾丸间质细胞，而是其他一些睾丸细胞类型，并且雌激素对猪睾丸间质细胞类固醇生成缺乏影响并非由于缺乏雌激素受体。
