Tabone E, Benahmed M, Reventos J, Saez J M
Cell Tissue Res. 1984;237(2):357-62. doi: 10.1007/BF00217157.
Interactions between Leydig and Sertoli cells, as well as a stimulatory effect of FSH on Leydig cell activity, have been reported in many studies. In order to investigate these interactions, the ultrastructure of immature pig Leydig cells under different culture conditions has been studied. When cultured alone in a chemically defined medium, there is a marked regression of the Leydig cell smooth endoplasmic reticulum and a swelling of the mitochondria. Addition of FSH or hCG does not prevent these phenomena. Co-culturing of Leydig cells with Sertoli cells from the same animal maintains the smooth endoplasmic reticulum at the level seen in vivo and in freshly isolated Leydig cells. The addition of FSH to the co-culture stimulates its development and increases Leydig cell activity, as assessed by an increase in hCG binding sites and an increased steroidogenic response to hCG. These results suggest that Sertoli cells exert a trophic effect on Leydig cells, and that the stimulatory effect of FSH on Leydig cell function is mediated via the Sertoli cells. These results reinforce the concept of a local regulatory control of Leydig cell steroidogenesis.
许多研究报道了睾丸间质细胞和支持细胞之间的相互作用，以及促卵泡激素（FSH）对睾丸间质细胞活性的刺激作用。为了研究这些相互作用，对不同培养条件下未成熟猪睾丸间质细胞的超微结构进行了研究。当在化学成分明确的培养基中单独培养时，睾丸间质细胞的滑面内质网会显著退化，线粒体肿胀。添加FSH或人绒毛膜促性腺激素（hCG）并不能阻止这些现象。将睾丸间质细胞与来自同一动物的支持细胞共培养，可使滑面内质网维持在体内和刚分离的睾丸间质细胞中所见的水平。在共培养体系中添加FSH可刺激其发育并增强睾丸间质细胞活性，这可通过hCG结合位点的增加以及对hCG的类固醇生成反应增强来评估。这些结果表明，支持细胞对睾丸间质细胞具有营养作用，且FSH对睾丸间质细胞功能的刺激作用是通过支持细胞介导的。这些结果强化了睾丸间质细胞类固醇生成的局部调节控制这一概念。
