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大肠杆菌K-12中的噬菌体λ受体蛋白：某些突变蛋白在体外对麦芽糖结合蛋白的亲和力降低。

Bacteriophage lambda receptor protein in Escherichia coli K-12: lowered affinity of some mutant proteins for maltose-binding protein in vitro.

作者信息

Luckey M, Nikaido H

出版信息

J Bacteriol. 1983 Feb;153(2):1056-9. doi: 10.1128/jb.153.2.1056-1059.1983.

DOI:10.1128/jb.153.2.1056-1059.1983
PMID:6218155
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC221731/
Abstract

Mutant and wild-type LamB proteins (phage lambda receptor proteins) were purified by affinity chromatography with immobilized maltose-binding protein, and their transport functions were tested in reconstituted liposomes. Two mutant proteins exhibited a marked decrease in affinity for immobilized maltose-binding protein, as well as altered transport rates.

摘要

通过使用固定化麦芽糖结合蛋白的亲和色谱法纯化突变型和野生型LamB蛋白（噬菌体λ受体蛋白），并在重构脂质体中测试它们的转运功能。两种突变蛋白对固定化麦芽糖结合蛋白的亲和力显著降低，并且转运速率也发生了改变。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cae5/221731/5052597c8fd9/jbacter00249-0488-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cae5/221731/e3b482d8a5f4/jbacter00249-0488-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cae5/221731/5052597c8fd9/jbacter00249-0488-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cae5/221731/e3b482d8a5f4/jbacter00249-0488-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cae5/221731/5052597c8fd9/jbacter00249-0488-b.jpg

相似文献

1
Bacteriophage lambda receptor protein in Escherichia coli K-12: lowered affinity of some mutant proteins for maltose-binding protein in vitro.大肠杆菌K-12中的噬菌体λ受体蛋白：某些突变蛋白在体外对麦芽糖结合蛋白的亲和力降低。
J Bacteriol. 1983 Feb;153(2):1056-9. doi: 10.1128/jb.153.2.1056-1059.1983.
2
The role of the periplasmic maltose-binding protein and the outer-membrane phage lambda receptor in maltodextrin transport of Escherichia coli.周质麦芽糖结合蛋白和外膜λ噬菌体受体在大肠杆菌麦芽糖糊精转运中的作用。
Biochem Soc Trans. 1980 Dec;8(6):680-1. doi: 10.1042/bst0080680.
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A mutant form of maltose-binding protein of Escherichia coli deficient in its interaction with the bacteriophage lambda receptor protein.一种大肠杆菌麦芽糖结合蛋白的突变形式，其与噬菌体λ受体蛋白的相互作用存在缺陷。
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Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli.噬菌体λ受体蛋白与大肠杆菌载体固定化麦芽糖结合蛋白之间的物理相互作用。
J Biol Chem. 1981 Nov 25;256(22):11385-8.
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The role of the Escherichia coli lambda receptor in the transport of maltose and maltodextrins.大肠杆菌λ受体在麦芽糖和麦芽糊精转运中的作用。
J Supramol Struct. 1980;13(1):101-16. doi: 10.1002/jss.400130110.
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Studies on the export of the maltose-binding protein and the LamB protein.麦芽糖结合蛋白和LamB蛋白的输出研究。
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The C-terminal portion of the tail fiber protein of bacteriophage lambda is responsible for binding to LamB, its receptor at the surface of Escherichia coli K-12.噬菌体λ尾丝蛋白的C末端部分负责与LamB结合，LamB是其在大肠杆菌K-12表面的受体。
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The maltose-maltodextrin transport system of Escherichia coli.大肠杆菌的麦芽糖-麦芽糊精转运系统
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引用本文的文献

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Maltose/maltodextrin system of Escherichia coli: transport, metabolism, and regulation.大肠杆菌的麦芽糖/麦芽糊精系统：转运、代谢与调控
Microbiol Mol Biol Rev. 1998 Mar;62(1):204-29. doi: 10.1128/MMBR.62.1.204-229.1998.
2
A mutant form of maltose-binding protein of Escherichia coli deficient in its interaction with the bacteriophage lambda receptor protein.一种大肠杆菌麦芽糖结合蛋白的突变形式，其与噬菌体λ受体蛋白的相互作用存在缺陷。
J Bacteriol. 1983 Aug;155(2):919-21. doi: 10.1128/jb.155.2.919-921.1983.
3
Monoclonal antibodies reveal lamB antigenic determinants on both faces of the Escherichia coli outer membrane.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Effect on solute size on diffusion rates through the transmembrane pores of the outer membrane of Escherichia coli.溶质大小对通过大肠杆菌外膜跨膜孔扩散速率的影响。
J Gen Physiol. 1981 Feb;77(2):121-35. doi: 10.1085/jgp.77.2.121.
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The role of the Escherichia coli lambda receptor in the transport of maltose and maltodextrins.大肠杆菌λ受体在麦芽糖和麦芽糊精转运中的作用。
单克隆抗体揭示了大肠杆菌外膜两面的lamB抗原决定簇。
J Bacteriol. 1983 Sep;155(3):1382-92. doi: 10.1128/jb.155.3.1382-1392.1983.
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Molecular basis of bacterial outer membrane permeability.细菌外膜通透性的分子基础。
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J Supramol Struct. 1980;13(1):101-16. doi: 10.1002/jss.400130110.
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Diffusion of solutes through channels produced by phage lambda receptor protein of Escherichia coli: inhibition by higher oligosaccharides of maltose series.溶质通过大肠杆菌噬菌体λ受体蛋白产生的通道的扩散：麦芽糖系列高级寡糖的抑制作用。
Biochem Biophys Res Commun. 1980 Mar 13;93(1):166-71. doi: 10.1016/s0006-291x(80)80261-0.
5
Physical interaction between the phage lambda receptor protein and the carrier-immobilized maltose-binding protein of Escherichia coli.噬菌体λ受体蛋白与大肠杆菌载体固定化麦芽糖结合蛋白之间的物理相互作用。
J Biol Chem. 1981 Nov 25;256(22):11385-8.
6
Nonspecific and specific diffusion channels in the outer membrane of Escherichia coli.大肠杆菌外膜中的非特异性和特异性扩散通道
J Supramol Struct. 1980;13(3):305-13. doi: 10.1002/jss.400130304.
7
Periplasmic maltose-binding protein confers specificity on the outer membrane maltose pore of Escherichia coli.周质麦芽糖结合蛋白赋予大肠杆菌外膜麦芽糖孔特异性。
J Bacteriol. 1980 Feb;141(2):431-5. doi: 10.1128/jb.141.2.431-435.1980.
8
Specificity of diffusion channels produced by lambda phage receptor protein of Escherichia coli.大肠杆菌λ噬菌体受体蛋白产生的扩散通道的特异性
Proc Natl Acad Sci U S A. 1980 Jan;77(1):167-71. doi: 10.1073/pnas.77.1.167.
9
Mutations that alter the transport function of the LamB protein in Escherichia coli.改变大肠杆菌中LamB蛋白转运功能的突变。
J Bacteriol. 1982 Jul;151(1):15-21. doi: 10.1128/jb.151.1.15-21.1982.
10
Gene sequence of the lambda receptor, an outer membrane protein of E. coli K12.λ受体的基因序列，大肠杆菌K12的一种外膜蛋白。
Cell. 1981 Dec;27(3 Pt 2):507-14. doi: 10.1016/0092-8674(81)90392-5.