Milon G, Marchal G, Seman M, Truffa-Bachi P, Zilberfarb V
J Immunol. 1983 Mar;130(3):1103-7.
In mice receiving, i.v., a dose of antigen optimal for antibody response, no delayed-type hypersensitivity (DTH) reaction is detectable. In contrast, in mice receiving a dose of antigen too small to induce B cell activation, a DTH reaction is elicitable shortly and transiently after immunization. Using a sensitive titration assay of DTH-mediating T lymphocytes, this reciprocal relationship between antibody production and DTH responses was reinvestigated. The absence of peripheral DTH reactivity in mice primed i.v. with a high dose of antigen (10(9) heterologous red blood cells) does not result either from the absence of activation and clonal expansion of DTH-mediating cells or from induction of suppressive mechanisms but results from a decreased circulation of DTH-mediating cells. The present studies show that DTH-mediating cells disappear from blood to enter the spleen only when specific B lymphocytes are present and activated by a high dose of antigen. These results are compatible with the hypothesis that T cells activated by antigen can function either as helper cells for B lymphocytes or as DTH-mediating cells, depending on the environment they reach during their migration. In order to demonstrate that the same cell may support the two functions, monoclonal T lymphocytes were assayed for their helper function and for their ability to transfer a DTH reaction.
给小鼠静脉注射对抗体反应而言最佳剂量的抗原时，检测不到迟发型超敏反应（DTH）。相反，给小鼠注射剂量过小而无法诱导B细胞活化的抗原时，免疫后不久可短暂诱发DTH反应。使用一种敏感的DTH介导性T淋巴细胞滴定测定法，对抗体产生与DTH反应之间的这种相互关系进行了重新研究。静脉注射高剂量抗原（10⁹个异源红细胞）致敏的小鼠外周缺乏DTH反应性，既不是因为缺乏DTH介导性细胞的活化和克隆扩增，也不是因为诱导了抑制机制，而是由于DTH介导性细胞的循环减少。目前的研究表明，只有当特异性B淋巴细胞存在并被高剂量抗原激活时，DTH介导性细胞才会从血液中消失并进入脾脏。这些结果与以下假设相符：被抗原激活的T细胞可作为B淋巴细胞的辅助细胞或作为DTH介导性细胞发挥作用，这取决于它们在迁移过程中到达的环境。为了证明同一细胞可能支持这两种功能，对单克隆T淋巴细胞的辅助功能及其传递DTH反应的能力进行了测定。
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