Knowles D M, Jakobiec F A
Am J Ophthalmol. 1983 Feb;95(2):233-42. doi: 10.1016/0002-9394(83)90019-3.
We performed indirect immunofluorescence with the OKT series of hybridoma monoclonal antibodies in order to determine the total number of T cells and their subset distribution, that is, the percentages of helper (OKT3+T4+T8-) and suppressor/cytotoxic (OKT3+T4-T8+) T cells, in 28 ocular adnexal lymphoid neoplasms. OKT3+T4+ (helper) T cells vastly predominated in each of ten benign, polyclonal ocular lymphoid proliferations. The helper-suppressor T-cell ratio (T4-8) ranged from 2.5 to 8.2 (mean, 5.4) in these ten cases. In comparison, the mean T4-T8 ratio in 24 benign reactive lymph nodes was 3.4. These findings strongly suggested that the polyclonal ocular lymphoid proliferations represent a T-cell antigen-dependent response characterized by a proliferation of helper T cells, which in turn drive B cells to proliferate and to differentiate, eventually resulting in the formation of a clinically detectable tumor. The mean T4-T8 ratio was 2.3 in 18 ocular and in 16 nodal monoclonal B-cell proliferations, suggesting that the benign T cells in these proliferations represent a residual cell population rather than a distinctive subset originating in response to the B-cell neoplasm.
我们用OKT系列杂交瘤单克隆抗体进行间接免疫荧光检测，以确定28例眼附属器淋巴样肿瘤中T细胞的总数及其亚群分布，即辅助性（OKT3+T4+T8-）和抑制/细胞毒性（OKT3+T4-T8+）T细胞的百分比。在10例良性多克隆性眼淋巴样增殖病变中，OKT3+T4+（辅助性）T细胞均占绝大多数。这10例病例中辅助性T细胞与抑制性T细胞的比例（T4/T8）为2.5至8.2（平均为5.4）。相比之下，24例良性反应性淋巴结的平均T4/T8比例为3.4。这些发现强烈提示，多克隆性眼淋巴样增殖病变代表一种T细胞抗原依赖性反应，其特征为辅助性T细胞增殖，进而促使B细胞增殖和分化，最终形成临床上可检测到的肿瘤。18例眼内和16例淋巴结单克隆B细胞增殖病变的平均T4/T8比例为2.3，提示这些增殖病变中的良性T细胞代表残留细胞群，而非因B细胞肿瘤而产生的独特亚群。
