Sharp T G, Sachs D H, Fauci A S, Messerschmidt G L, Rosenberg S A
Transplantation. 1983 Feb;35(2):112-20. doi: 10.1097/00007890-198302000-00002.
Human bone marrow was harvested from surgically resected bones of 25 patients and was tested for the presence of mature T cells. An average of 6.5% (+/- 1.2% SE) of nucleated bone marrow cells formed spontaneous rosettes with sheep red blood cells. Functional T cells in bone marrow were also identified by characteristic responses to alloantigens and the T cell mitogens concanavalin A (Con A) and phytohemagglutinin (PHA). The ability of three monoclonal antibodies (OKT.3, Lyt-3, and (Leu-1) to lyse peripheral T cells in the presence of rabbit complement was examined. All three reagents were found to be specifically lytic for mature T cells in peripheral blood. One reagent (Leu-1) was selected for use in depletion of T cells in human bone marrow. Seven of 10 experiments performed showed sufficient T cell responses to be evaluable. In all of these experiments, a marked reduction of T cells and T cell functions was observed. On the average, E rosettes were reduced 89.2% (+/- 3.0% SE) below medium controls while the mean PHA, Con A, and mixed lymphocyte culture (MLC) activity were completely eliminated to levels below background. In four experiments, colony-forming units (CFU-GM) in bone marrow were assayed following treatment with Leu-1 and showed a mean increase of 194% (+/- 32% SE) over medium controls. Since mature T cells are thought to be responsible for graft-versus-host disease in allogeneic bone marrow transplantation, this method of T cell depletion may be useful for preparing marrow for human bone marrow transplants.
从25例患者手术切除的骨骼中采集人骨髓，并检测其中成熟T细胞的存在情况。平均6.5%（±1.2%标准误）的有核骨髓细胞与绵羊红细胞形成自发玫瑰花结。骨髓中的功能性T细胞还通过对同种异体抗原以及T细胞有丝分裂原刀豆球蛋白A（Con A）和植物血凝素（PHA）的特征性反应来鉴定。检测了三种单克隆抗体（OKT.3、Lyt-3和（Leu-1））在兔补体存在下裂解外周血T细胞的能力。发现所有这三种试剂对外周血中的成熟T细胞均具有特异性裂解作用。选择一种试剂（Leu-1）用于去除人骨髓中的T细胞。所进行的10项实验中有7项显示有足够的T细胞反应可供评估。在所有这些实验中，均观察到T细胞和T细胞功能明显降低。平均而言，E玫瑰花结比培养基对照降低了89.2%（±3.0%标准误），而PHA、Con A和混合淋巴细胞培养（MLC）的平均活性则完全消除至背景水平以下。在四项实验中，用Leu-1处理后检测了骨髓中的集落形成单位（CFU-GM），结果显示比培养基对照平均增加了194%（±32%标准误）。由于成熟T细胞被认为是同种异体骨髓移植中移植物抗宿主病的病因，这种T细胞去除方法可能有助于为人类骨髓移植准备骨髓。
