Brasitus T A
Biochim Biophys Acta. 1983 Feb 9;728(1):20-30. doi: 10.1016/0005-2736(83)90432-7.
Lipid dynamics and lipid-protein interactions were examined in basolateral membranes prepared from rat proximal and distal colonic epithelial cells. The results demonstrate that: (1) these membranes have a high lipid fluidity, as assessed by steady-state fluorescence polarization studies using seven fluorescent probes; (2) lipid compositional differences exist between these membranes but their fluidity is similar; (3) fluorescence polarization studies, using diphenylhexatriene (DPH), detect a thermotropic transition at 22-23 degrees C in each membrane; (4) several membrane protein activities, including adenylate cyclase and sodium-potassium dependent adenosine triphosphatase ((Na+ + K+)-ATPase) appear to be functionally dependent on the physical state of the proximal basolateral membrane's lipid.
在从大鼠近端和远端结肠上皮细胞制备的基底外侧膜中研究了脂质动力学和脂质 - 蛋白质相互作用。结果表明：（1）通过使用七种荧光探针的稳态荧光偏振研究评估，这些膜具有高脂质流动性；（2）这些膜之间存在脂质组成差异，但它们的流动性相似；（3）使用二苯基己三烯（DPH）的荧光偏振研究在每个膜中检测到22 - 23摄氏度的热致转变；（4）几种膜蛋白活性，包括腺苷酸环化酶和钠钾依赖性腺苷三磷酸酶（（Na + + K +）-ATP酶），似乎在功能上依赖于近端基底外侧膜脂质的物理状态。
