Curatola G, Fiorini R M, Solaini G, Baracca A, Parenti Castelli G, Lenaz G
FEBS Lett. 1983 May 2;155(1):131-4. doi: 10.1016/0014-5793(83)80224-5.
Isolated oligomycin-sensitive ATPase undergoes a kinetic change at 20-25 degrees C with a higher activation energy and a lower Km for ATP below this temperature range. This observation has been correlated with temperature-dependent structural changes detected by circular dichroism in the UV region in the isolated enzyme. The negative ellipticities in the 208-225 nm region, which are proportional to the alpha-helix content, increase with rise in temperature to a maximum above 25 degrees C.
分离出的寡霉素敏感ATP酶在20-25摄氏度时发生动力学变化，在此温度范围以下，其具有较高的活化能和较低的ATP米氏常数。这一观察结果与通过圆二色性在紫外区域检测到的该分离酶中温度依赖性结构变化相关。在208-225纳米区域的负椭圆率与α-螺旋含量成正比，随温度升高而增加，在25摄氏度以上达到最大值。
