Nagase F, Nakashima I, Kato N
Dev Comp Immunol. 1983 Winter;7(1):127-37. doi: 10.1016/0145-305x(83)90061-7.
The role of carrier recognition in the hapten-specific antibody response of chickens to hapten (TNP) conjugate of sheep red blood cells (SRBC) was studied. The anti-TNP antibody response to the challenge with either TNP-SRBC or DNP conjugate of Salmonella enteritidis (DNP-Sal) of chickens previously primed with TNP-SRBC was definitely higher than that of unprimed chickens. Injection of TNP-chicken red blood cells, which did not provoke any significant primary anti-TNP response, also induced the memory for secondary anti-TNP response to the challenge with TNP-SRBC or DNP-Sal. The TNP-specific memory generated by stimulation with TNP-SRBC was adoptively transferable by spleen cells into cyclophosphamide (CY)-treated X-irradiated syngeneic recipients. On the other hand, the SRBC-specific memory for augmentation of anti-TNP response to the challenge with TNP-SRBC was never detected in chickens previously primed with either any of a wide range of doses (10(4)-10(8)) of SRBC, solubilized SRBC or SRBC fixed with formaldehyde, nor in CY-treated X-irradiated chickens receiving SRBC-primed spleen cells. The anti-TNP response of chickens to the challenge with TNP-SRBC was rather suppressed by the SRBC preimmunization. The passively administered anti-SRBC chicken serum also suppressed the response to TNP-SRBC. The carrier-specific suppressor activity generated by SRBC stimulation was not adoptively transferable by spleen cells. It is suggested from these results that the mechanism of amplification of the anti-hapten antibody response of chickens to hapten-SRBC conjugate is primarily mediated by the hapten-recognition, and carrier-recognition is more important for suppression rather than for amplification of the anti-hapten response, although the inductive mechanism for anti-hapten response itself should somehow involve the carrier recognition.
研究了载体识别在鸡对羊红细胞（SRBC）的半抗原（TNP）缀合物的半抗原特异性抗体应答中的作用。先前用TNP-SRBC免疫的鸡，在用TNP-SRBC或肠炎沙门氏菌的DNP缀合物（DNP-Sal）攻击时，其抗TNP抗体应答明显高于未免疫的鸡。注射TNP-鸡红细胞，虽然不会引发任何显著的初次抗TNP应答，但也能诱导对TNP-SRBC或DNP-Sal攻击的二次抗TNP应答记忆。用TNP-SRBC刺激产生的TNP特异性记忆可通过脾细胞过继转移至经环磷酰胺（CY）处理并接受X射线照射的同基因受体。另一方面，在用各种剂量（10⁴ - 10⁸）的SRBC、溶解的SRBC或甲醛固定的SRBC免疫的鸡中，从未检测到对TNP-SRBC攻击增强抗TNP应答的SRBC特异性记忆，在接受SRBC免疫的脾细胞的经CY处理并接受X射线照射的鸡中也未检测到。SRBC预免疫反而抑制了鸡对TNP-SRBC攻击的抗TNP应答。被动给予的抗SRBC鸡血清也抑制了对TNP-SRBC的应答。SRBC刺激产生的载体特异性抑制活性不能通过脾细胞过继转移。从这些结果表明，鸡对半抗原-SRBC缀合物的抗半抗原抗体应答放大机制主要由半抗原识别介导，载体识别对于抗半抗原应答的抑制比放大更重要，尽管抗半抗原应答本身的诱导机制在某种程度上应涉及载体识别。
