Selitrennikoff C P
Dev Biol. 1983 May;97(1):245-9. doi: 10.1016/0012-1606(83)90082-9.
Cell wall formation of Neurospora crassa and other filamentous fungi involves the apical extension of preexisting cell wall in a complex assembly sequence; however, it is not known if preexisting wall participates in the formation of new cell wall. It was found that temperature-sensitive protoplasts which lack detectable preexisting wall form cell wall upon a shift to a permissive temperature. Similarly, temperature-sensitive colonial mutants form morphologically normal cell wall directly from preexisting abnormal hyphae after a shift to a permissive temperature. These results are consistent with the idea that cell wall assembly occurs without the participation of preexisting cell wall as either primer or template for new cell wall assembly.
粗糙脉孢菌及其他丝状真菌的细胞壁形成涉及已有细胞壁在复杂组装序列中的顶端延伸；然而，尚不清楚已有细胞壁是否参与新细胞壁的形成。研究发现，缺乏可检测到的已有细胞壁的温度敏感型原生质体在转移至允许温度时会形成细胞壁。同样，温度敏感型菌落突变体在转移至允许温度后，能直接从已有的异常菌丝形成形态正常的细胞壁。这些结果与以下观点一致，即细胞壁组装在没有已有细胞壁作为新细胞壁组装的引物或模板参与的情况下发生。
