Modlin R L, Hofman F M, Meyer P R, Sharma O P, Taylor C R, Rea T H
Clin Exp Immunol. 1983 Mar;51(3):430-8.
T lymphocyte subpopulations in frozen tissue sections of four granulomatous conditions (five patients with tuberculoid leprosy, five with lepromatous leprosy, seven with sarcoidosis and four with rhinoscleroma) were studied using monoclonal antibodies and a modified immunoperoxidase technique. Two immunohistological patterns were observed. In tuberculoid leprosy and sarcoidosis, lymphocytes expressing the helper/inducer phenotype were present within the aggregates of mononuclear phagocytes (epithelioid cells); however, cells with the suppressor/cytotoxic phenotype were predominantly in the lymphocytic mantle surrounding each granuloma. In lepromatous leprosy and rhinoscleroma the helper/inducer T cells and suppressor/cytotoxic T cells were both diffusely distributed among the mononuclear phagocytes (histiocytes) without any discernible mantle. The segregation of the helper/inducer and suppressor/cytotoxic phenotypic subsets was associated with an epithelioid cell differentiation of mononuclear phagocytic cells, bacterial elimination and a delayed type hypersensitivity response. The intimate admixture of helper/inducer and suppressor/cytotoxic subsets was associated with undifferentiated mononuclear phagocytes, bacterial proliferation and the absence of a delayed type hypersensitivity response. Thus the different distributions of T cell subpopulations in granulomas may be associated with differences in the host's immune response in several forms of granulomatous reactions.
采用单克隆抗体和改良免疫过氧化物酶技术，对4种肉芽肿性疾病（5例结核样型麻风、5例瘤型麻风、7例结节病和4例鼻硬结病）冷冻组织切片中的T淋巴细胞亚群进行了研究。观察到两种免疫组织学模式。在结核样型麻风病和结节病中，表达辅助/诱导表型的淋巴细胞存在于单核吞噬细胞（上皮样细胞）聚集体中；然而，具有抑制/细胞毒性表型的细胞主要位于每个肉芽肿周围的淋巴细胞套中。在瘤型麻风病和鼻硬结病中，辅助/诱导性T细胞和抑制/细胞毒性T细胞均弥漫分布于单核吞噬细胞（组织细胞）之间，无明显的套区。辅助/诱导表型亚群和抑制/细胞毒性表型亚群的分离与单核吞噬细胞的上皮样细胞分化、细菌清除及迟发型超敏反应相关。辅助/诱导亚群和抑制/细胞毒性亚群的紧密混合与未分化的单核吞噬细胞、细菌增殖及迟发型超敏反应的缺乏相关。因此，肉芽肿中T细胞亚群的不同分布可能与几种肉芽肿反应中宿主免疫反应的差异有关。
