噬菌体φX174基因C蛋白的纯化

Purification of phi X174 gene C protein.

作者信息

Aoyama A, Hamatake R K, Mukai R, Hayashi M

出版信息

J Biol Chem. 1983 May 10;258(9):5798-803.

PMID:6222055

Abstract

The product of gene C of bacteriophage phi X174 is required for the replication of phiX174 single-stranded DNA in Escherichia coli cells infected with phi X174. The protein has been purified to homogeneity using an in vitro complementation system. The protein exhibits a molecular weight of 5800 under denaturing conditions. The NH2-terminal amino acid sequence of the protein is (NH2)-Met-Arg-Lys, which is consistent with the sequence predicted from the nucleotide sequence of gene C. The protein has an affinity for single-stranded DNA but less for double-stranded DNA.

摘要

噬菌体φX174的基因C产物是φX174单链DNA在感染了φX174的大肠杆菌细胞中复制所必需的。该蛋白质已使用体外互补系统纯化至同质。在变性条件下，该蛋白质的分子量为5800。该蛋白质的NH2末端氨基酸序列为(NH2)-Met-Arg-Lys，这与从基因C的核苷酸序列预测的序列一致。该蛋白质对单链DNA有亲和力，但对双链DNA的亲和力较小。