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噬菌体φX174基因A蛋白的识别序列——DNA复制起始因子

Recognition sequence of bacteriophage phi X174 gene A protein--an initiator of DNA replication.

作者信息

van Mansfeld A D, Langeveld S A, Baas P D, Jansz H S, van der Marel G A, Veeneman G H, van Boom J H

出版信息

Nature. 1980 Dec 11;288(5791):561-6. doi: 10.1038/288561a0.

DOI:10.1038/288561a0
PMID:6255338
Abstract

Gene A protein, the initiator protein of bacteriophage phi X174 DNA replication, cleaves synthetic single-stranded oligodeoxyribonucleotides at the same site as the corresponding sequence at the phi X origin. The results identify the recognition sequence within the decamer CAACTTGATA which is cleaved next to the G residue. Further requirements for cleavage of double-stranded DNA by the gene A protein are supercoiling and an A + T-rich domain adjacent to the recognition sequence.

摘要

基因A蛋白是噬菌体φX174 DNA复制的起始蛋白，它在与φX起始点相应序列相同的位点切割合成单链寡脱氧核糖核苷酸。结果确定了十聚体CAACTTGATA内的识别序列，该序列在G残基旁边被切割。基因A蛋白切割双链DNA的进一步要求是超螺旋以及识别序列附近富含A+T的区域。

相似文献

1
Recognition sequence of bacteriophage phi X174 gene A protein--an initiator of DNA replication.噬菌体φX174基因A蛋白的识别序列——DNA复制起始因子
Nature. 1980 Dec 11;288(5791):561-6. doi: 10.1038/288561a0.
2
Gene A protein of bacteriophage phi X174 is a highly specific single-strand nuclease and binds via a tyrosyl residue to DNA after cleavage.噬菌体φX174的基因A蛋白是一种高度特异性的单链核酸酶，切割后通过一个酪氨酰残基与DNA结合。
Adv Exp Med Biol. 1984;179:221-30. doi: 10.1007/978-1-4684-8730-5_23.
3
The bond in the bacteriophage phi X174 gene A protein--DNA complex is a tyrosyl-5'-phosphate ester.噬菌体φX174基因A蛋白与DNA复合物中的键是酪氨酰-5'-磷酸酯。
FEBS Lett. 1984 Aug 6;173(2):351-6. doi: 10.1016/0014-5793(84)80804-2.
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The nuclease specificity of the bacteriophage phi X174 A* protein.噬菌体φX174 A*蛋白的核酸酶特异性
Nucleic Acids Res. 1981 Feb 11;9(3):545-62. doi: 10.1093/nar/9.3.545.
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Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication.含有噬菌体φX174 DNA复制起点序列的重组质粒DNA的构建与特性分析。
Nucleic Acids Res. 1981 Jul 24;9(14):3335-54. doi: 10.1093/nar/9.14.3335.
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Gene A protein cleavage of recombinant plasmids containing the phi X174 replication origin.含有φX174复制起点的重组质粒的基因A蛋白切割
Nucleic Acids Res. 1984 Aug 24;12(16):6443-54. doi: 10.1093/nar/12.16.6443.
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Effect of SSB protein on cleavage of single-stranded DNA by phi X gene A protein and A* protein.SSB蛋白对φX基因A蛋白和A*蛋白切割单链DNA的影响。
Nucleic Acids Res. 1986 Feb 25;14(4):1845-61. doi: 10.1093/nar/14.4.1845.
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In vitro DNA replication of recombinant plasmid DNAs containing the origin of progeny replicative form DNA synthesis of phage phi X174.含有噬菌体φX174子代复制型DNA合成起始位点的重组质粒DNA的体外DNA复制。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5182-6. doi: 10.1073/pnas.77.9.5182.
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Mutational analysis of the bacteriophage phi X174 replication origin.噬菌体φX174复制起点的突变分析
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Termination and reinitiation signals of bacteriophage phi X174 rolling circle DNA replication.噬菌体φX174滚环DNA复制的终止和重新起始信号
Virology. 1986 Oct 30;154(2):357-68. doi: 10.1016/0042-6822(86)90461-7.

引用本文的文献

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Nucleic Acids Res. 2000 Jun 1;28(11):2251-5. doi: 10.1093/nar/28.11.2251.
2
DNA methylation inhibits the transfecting activity of replicative- form phi X174 DNA.DNA甲基化抑制复制型φX174 DNA的转染活性。
J Virol. 1984 Mar;49(3):674-9. doi: 10.1128/JVI.49.3.674-679.1984.
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DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications.原核生物和真核生物中的DNA聚合酶：作用模式及生物学意义
Experientia. 1983 Jan 15;39(1):1-25. doi: 10.1007/BF01960616.
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Initiation and termination of the bacteriophage phi X174 rolling circle DNA replication in vivo: packaging of plasmid single-stranded DNA into bacteriophage phi X174 coats.噬菌体φX174滚环DNA复制在体内的起始与终止：质粒单链DNA包装进噬菌体φX174衣壳
Nucleic Acids Res. 1982 Nov 11;10(21):6849-63. doi: 10.1093/nar/10.21.6849.
5
Nucleotide sequences at the phi X gene A protein cleavage site in replicative form I DNAs of bacteriophages U3, G14, and alpha 3.噬菌体U3、G14和α3的复制型I DNA中X基因A蛋白切割位点的核苷酸序列。
J Virol. 1982 Apr;42(1):91-9. doi: 10.1128/JVI.42.1.91-99.1982.
6
On the molecular mechanisms of transposition.关于转座的分子机制
Proc Natl Acad Sci U S A. 1981 Aug;78(8):4858-62. doi: 10.1073/pnas.78.8.4858.
7
Construction and characterization of recombinant plasmid DNAs containing sequences of the origin of bacteriophage phi X174 DNA replication.含有噬菌体φX174 DNA复制起点序列的重组质粒DNA的构建与特性分析。
Nucleic Acids Res. 1981 Jul 24;9(14):3335-54. doi: 10.1093/nar/9.14.3335.
8
Gene A protein cleavage of recombinant plasmids containing the phi X174 replication origin.含有φX174复制起点的重组质粒的基因A蛋白切割
Nucleic Acids Res. 1984 Aug 24;12(16):6443-54. doi: 10.1093/nar/12.16.6443.
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Mol Gen Genet. 1982;187(1):162-5. doi: 10.1007/BF00384400.
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Proc Natl Acad Sci U S A. 1982 Dec;79(24):7724-8. doi: 10.1073/pnas.79.24.7724.