虹鳟（Salmo gairdneri R.）糖酵解的营养调控

Nutritional regulation of glycolysis in rainbow trout (Salmo gairdneri R.).

Fideu M D, Soler G, Ruiz-Amil M

Comp Biochem Physiol B. 1983;74(4):795-9. doi: 10.1016/0305-0491(83)90148-7.

Abstract

Some physico-chemical constants and the nutritional regulation of pyruvate kinase (PK), phosphofructokinase (PFK) and hexokinase (HK) from rainbow trout liver was investigated. 2. The maximum activity pH for the three enzymes appears to be in a physiological range. 3. The PK-enzyme shows sigmoid kinetic with respect to PEP with a Hill-coefficient of 3.1; the other two enzymes show michaelian kinetic for their substrates. 4. The nutritional treatments show that HK-enzyme increases its level with high carbohydrate diet and decreases with high protein diet and starvation. 5. PFK-enzyme decreases with high protein diet and starvation. 6. PK-enzyme only shows a decrease in level with starvation conditions.

摘要

研究了虹鳟鱼肝中丙酮酸激酶（PK）、磷酸果糖激酶（PFK）和己糖激酶（HK）的一些物理化学常数及其营养调控。2. 这三种酶的最大活性pH似乎处于生理范围内。3. PK酶对磷酸烯醇式丙酮酸（PEP）呈现S形动力学，希尔系数为3.1；另外两种酶对其底物呈现米氏动力学。4. 营养处理表明，HK酶在高碳水化合物饮食时水平升高，在高蛋白饮食和饥饿时水平降低。5. PFK酶在高蛋白饮食和饥饿时降低。6. PK酶仅在饥饿条件下水平降低。