Gimeno A
J Assoc Off Anal Chem. 1983 May;66(3):565-9.
A rapid method is described for determining zearalenone in corn, sorghum, and wheat. The mycotoxin is extracted with a mixture of acetonitrile and 4% KCl in HCl. The extract is cleaned up with isooctane, evaporated, and redissolved in chloroform. Zearalenone is separated by thin layer chromatography; identity is confirmed with various developing solvents and spray reagents. Zearalenone is then quantitated by the limit detection method. The minimum detectable concentration is 140-160 micrograms/kg when aluminum chloride solution is used as spray reagent, and 85-110 micrograms/kg when Fast Violet B salt is used as spray reagent.
本文描述了一种快速测定玉米、高粱和小麦中玉米赤霉烯酮的方法。用乙腈和4%氯化钾的盐酸混合液提取霉菌毒素。提取物用异辛烷净化，蒸发后再溶解于氯仿中。通过薄层色谱法分离玉米赤霉烯酮；用各种展开溶剂和喷雾试剂确认其身份。然后用限量检测法对玉米赤霉烯酮进行定量。当使用氯化铝溶液作为喷雾试剂时，最低可检测浓度为140 - 160微克/千克；当使用固紫B盐作为喷雾试剂时，最低可检测浓度为85 - 110微克/千克。
