用粗糙脉孢菌和鸟类红细胞的膜提取物重建激素敏感型腺苷酸环化酶。

Reconstitution of a hormone-sensitive adenylate cyclase with membrane extracts from Neurospora and avian erythrocytes.

作者信息

Flawiá M M, Kornblihtt A R, Reig J A, Torruella M, Torres H N

出版信息

J Biol Chem. 1983 Jul 10;258(13):8255-9.

Abstract

Adenylate cyclase activity associated to wild type Neurospora membranes is highly dependent on Mn2+ and insensitive to fluoride, guanyl nucleotides, and cholera toxin. These membranes are able to interact with components of detergent extracts from turkey erythrocyte ghosts. The reconstituted cyclase system is catalytically active in the presence of Mg2+ and it is activated by guanyl-5'-yl imidodiphosphate plus isoproterenol and fluoride. When detergent extracts were prepared from avian erythrocyte membranes treated with cholera toxin, the reconstituted system was stimulated by guanyl-5'-yl imidodiphosphate in the absence of isoproterenol and cyclase activities were higher than those observed with extracts from membranes not treated with the toxin. Dose-response curves for isoproterenol and fluoride in the reconstituted system were similar to those reported for avian erythrocyte and liver membranes, respectively.

摘要

与野生型脉孢菌细胞膜相关的腺苷酸环化酶活性高度依赖于Mn2+，且对氟化物、鸟苷核苷酸和霍乱毒素不敏感。这些细胞膜能够与来自火鸡红细胞血影的去污剂提取物成分相互作用。重构的环化酶系统在Mg2+存在时具有催化活性，并且被鸟苷-5'-基亚氨基二磷酸加异丙肾上腺素和氟化物激活。当从用霍乱毒素处理的禽红细胞膜制备去污剂提取物时，重构系统在没有异丙肾上腺素的情况下被鸟苷-5'-基亚氨基二磷酸刺激，并且环化酶活性高于从未用该毒素处理的膜提取物中观察到的活性。重构系统中异丙肾上腺素和氟化物的剂量反应曲线分别与禽红细胞和肝细胞膜报道的曲线相似。