Eisenschlos C D, Paladini A A, Molina y Vedia L, Torres H N, Flawiá M M
Biochem J. 1986 Aug 1;237(3):913-7. doi: 10.1042/bj2370913.
The existence of a GTP-binding protein of the Ns type in Trypanosoma cruzi was explored. Epimastigote membranes were labelled by cholera toxin in the presence of [adenine-14C]NAD+. After SDS/polyacrylamide-gel electrophoresis of extracted membrane proteins, a single labelled polypeptide band of apparent Mr approx. 45,000 was detected. Epimastigote cells were treated with N-ethylmaleimide and electrofused to lymphoma S49 cells lacking the Ns protein. Evidence indicates that in such electrofusion-generated cell hybrids a heterologous adenylate cyclase system was reconstituted with the Ns protein provided by T. cruzi epimastigotes.
对克氏锥虫中Ns型GTP结合蛋白的存在进行了探索。在[腺嘌呤-14C]NAD+存在的情况下，用霍乱毒素标记无鞭毛体膜。对提取的膜蛋白进行SDS/聚丙烯酰胺凝胶电泳后，检测到一条表观分子量约为45,000的单一标记多肽带。用N-乙基马来酰亚胺处理无鞭毛体细胞，并将其电融合到缺乏Ns蛋白的淋巴瘤S49细胞中。有证据表明，在这种电融合产生的细胞杂种中，克氏锥虫无鞭毛体提供的Ns蛋白重组了一个异源腺苷酸环化酶系统。
