Nagarkatti P S, Clark D A
J Immunol. 1983 Aug;131(2):638-43.
The fetus that results from allogeneic mating in an outbred population bears a variety of antigens against which the maternal immune system reacts, but the type of immunity that is elicited by pregnancy does not mediate graft rejection. Previous studies have demonstrated the presence of nonspecific non-thymus derived suppressor cells in the lymph nodes draining the uterus (DLN) and in the uterine decidua during first allogeneic pregnancy. These suppressor cells appear to be small lymphoid cells that inhibit the generation of cytotoxic T cells (CTL) against paternal alloantigens. We now report that after a single allogeneic pregnancy, C3H and A strain mice develop paternal alloantigen-specific suppressor T cells (Thy-1.2+, Lyt-1-2+) that are distributed systemically in peripheral lymph nodes and spleen. These suppressor cells appear to act directly on CTL generation because the frequency of CTL precursors and the ability to produce T helper cells in response to paternal alloantigens remains unchanged after allogeneic pregnancy. Suppressor T cell activity could be detected both in vitro and in vivo, and the presence of suppression in vivo was associated with increased uterine weight 5 days after inoculation of allogeneic paternal tumor cells into the uterus of pseudopregnant mice. Nevertheless, both subcutaneous and intrauterine tumor inocula regressed in these mice with suppressor T cells. The possible role of suppressor T cells in contrast to non-T suppressor cells in successful reproduction and allopregnancy-induced tolerance is discussed.
在远交群体中，异体交配产生的胎儿带有多种抗原，母体免疫系统会对这些抗原产生反应，但怀孕引发的免疫类型不会介导移植排斥反应。先前的研究表明，在首次异体妊娠期间，子宫引流淋巴结（DLN）和子宫蜕膜中存在非特异性非胸腺来源的抑制细胞。这些抑制细胞似乎是小型淋巴细胞，可抑制针对父本同种异体抗原的细胞毒性T细胞（CTL）的生成。我们现在报告，单次异体妊娠后，C3H和A品系小鼠会产生父本同种异体抗原特异性抑制性T细胞（Thy-1.2 +，Lyt-1-2 +），这些细胞全身分布在外周淋巴结和脾脏中。这些抑制细胞似乎直接作用于CTL的生成，因为异体妊娠后，CTL前体细胞的频率以及对父本同种异体抗原产生辅助性T细胞的能力保持不变。抑制性T细胞活性在体外和体内均可检测到，在假孕小鼠子宫内接种异体父本肿瘤细胞5天后，体内抑制作用的存在与子宫重量增加有关。然而，在这些具有抑制性T细胞的小鼠中，皮下和子宫内接种的肿瘤均会消退。本文讨论了抑制性T细胞与非T抑制细胞相比在成功繁殖和同种异体妊娠诱导的耐受性中的可能作用。
