从正常鸡和营养不良鸡的同基因系中分离出的肌浆网中的钙转运、ATP酶活性和脂质组成。

Calcium transport, ATPase activity and lipid composition in sarcoplasmic reticulum isolated from isogenic lines of normal and dystrophic chickens.

作者信息

Kawamoto R M, Baskin R J

出版信息

Biochim Biophys Acta. 1983 Aug 10;732(3):620-6. doi: 10.1016/0005-2736(83)90239-0.

Abstract

Two new lines of chickens with near identical genotypes (greater than 90% isogeneity), one demonstrating avian dystrophy, were used for isolation of sarcoplasmic reticulum vesicles. Vesicles from line 433 (dystrophic) displayed reduced Ca2+-ATPase activity, phosphoenzyme formation and steady-state calcium transport capabilities in comparison with vesicles from line 03 (normal). Lipid analyses show that dystrophic vesicles have greater amounts of cholesterol and lesser amounts of phosphatidylcholine. The results support the use of isogenic chickens in further studies of avian dystrophy. However, the results also suggest that current sarcoplasmic reticulum vesicle purification procedures dependent on differential calcium accumulation may not fully achieve the intended purpose.

摘要

使用了两个具有近乎相同基因型（同质性大于90%）的新鸡系，其中一个表现出禽类营养不良，用于分离肌浆网囊泡。与03系（正常）的囊泡相比，433系（营养不良）的囊泡显示出Ca2+-ATP酶活性、磷酸酶形成和稳态钙转运能力降低。脂质分析表明，营养不良的囊泡含有更多的胆固醇和更少的磷脂酰胆碱。这些结果支持在禽类营养不良的进一步研究中使用同基因鸡。然而，结果也表明，目前依赖于差异钙积累的肌浆网囊泡纯化程序可能无法完全达到预期目的。