Volpi L, Ghisotti D, Sironi G
Virology. 1983 Jul 15;128(1):166-75. doi: 10.1016/0042-6822(83)90327-6.
Escherichia coli cells carrying lambda cI857 prophage lyse 40 min after lambda thermoinduction; the lysis depends on the lambda genes Q, R, and S. If chloramphenicol (CAP) is added within 20 min after lambda cI857 induction, an early, unproductive lysis occurs. This lysis is independent of the genes int, rex, O, P, Q, and all late genes. Instead, early lysis depends upon the kil gene. The early lysis is under the positive control of lambda gene N and the negative control of gene cro. One or more events specifically connected with lambda induction appear to be necessary for the occurrence of early lysis, since early lysis cannot be observed after lambda infection. Induced lambda kil+ lysogens are more sensitive to osmotic shock than induced lambda kil- lysogens. CAP-induced early lysis can be prevented in a hypertonic medium. These results suggest that induction of lambda causes an osmotic fragility due to a damage of the cell envelope which requires repair; in the absence of protein synthesis the cell envelope is not repaired and cell lysis ensues.
携带λcI857原噬菌体的大肠杆菌细胞在λ热诱导后40分钟裂解；这种裂解依赖于λ基因Q、R和S。如果在λcI857诱导后20分钟内加入氯霉素（CAP），则会发生早期的、无效的裂解。这种裂解不依赖于int、rex、O、P、Q基因以及所有晚期基因。相反，早期裂解依赖于kil基因。早期裂解受λ基因N的正调控和cro基因的负调控。早期裂解的发生似乎需要一个或多个与λ诱导特别相关的事件，因为在λ感染后观察不到早期裂解。诱导的λkil +溶原菌比诱导的λkil -溶原菌对渗透压休克更敏感。在高渗培养基中可以防止CAP诱导的早期裂解。这些结果表明，λ的诱导会由于细胞膜的损伤而导致渗透压脆性，这种损伤需要修复；在没有蛋白质合成的情况下，细胞膜无法修复，细胞随之裂解。
