Peterson R N, Ashraf M, Russell L D
Biochem Biophys Res Commun. 1983 Jul 18;114(1):28-33. doi: 10.1016/0006-291x(83)91589-9.
Calcium uptake by washed boar sperm suspensions is markedly stimulated by the calmodulin antagonists trifluoperazine and calmidazolium. Both 45Ca2+ uptake and net Ca2+ uptake are increased by these drugs. Drug stimulated Ca2+ uptake is blocked by verapamil (1 mM), by ruthenium red (25 microM) and by carbonyl cyanide p-trifluoromethoxyphenyl hydrazone. Calmodulin antagonists do not slow ATP-dependent Ca2+ extrusion from plasma membrane vesicles, and they do not inhibit plasma membrane Ca2+-ATPase. It is proposed that calmodulin is involved in the control of Ca2+ entry in boar spermatozoa. Most entering Ca2+ in uncapacitated spermatozoa is sequestered by mitochondria or rapidly extruded by plasma membrane pumps. In contrast to the uptake mechanism, ATP-dependent Ca2+ extrusion does not appear to be regulated by calmodulin.
钙调蛋白拮抗剂三氟拉嗪和氯咪巴唑可显著刺激洗涤后的公猪精子悬液对钙的摄取。这两种药物均可增加45Ca2+摄取量和净Ca2+摄取量。药物刺激的Ca2+摄取可被维拉帕米（1 mM）、钌红（25 microM）和羰基氰对三氟甲氧基苯腙阻断。钙调蛋白拮抗剂不会减缓质膜囊泡中ATP依赖的Ca2+外排，也不会抑制质膜Ca2+-ATP酶。有人提出钙调蛋白参与公猪精子中Ca2+进入的控制。未获能精子中大多数进入的Ca2+被线粒体隔离或被质膜泵迅速排出。与摄取机制不同，ATP依赖的Ca2+外排似乎不受钙调蛋白调节。
