Tanaka A, Morita J, Komano T
J Antibiot (Tokyo). 1983 Jul;36(7):900-6. doi: 10.7164/antibiotics.36.900.
Aclacinomycin A inhibited the in vitro conversion of phage phi X174 single-stranded DNA to the replicative form DNA. DNA synthesis was inhibited by 50% in the presence of 15 microM aclacinomycin A. The inhibition was competitive with respect to template DNA (Ki = 13 microM) and was reversed by addition of Escherichia coli cell extracts. Short complementary strands approximately one-third of unit length molecule were synthesized in the presence of 15 microM aclacinomycin A. The data suggest that aclacinomycin A may inhibit the process of phi X174 DNA chain elongation by a direct interaction with the E. coli host enzymes.
阿克拉霉素A抑制了噬菌体φX174单链DNA在体外转化为复制型DNA的过程。在存在15微摩尔阿克拉霉素A的情况下，DNA合成被抑制了50%。这种抑制作用在模板DNA方面具有竞争性（抑制常数Ki = 13微摩尔），并且通过添加大肠杆菌细胞提取物可以逆转。在存在15微摩尔阿克拉霉素A的情况下，合成了大约为单位长度分子三分之一的短互补链。数据表明，阿克拉霉素A可能通过与大肠杆菌宿主酶直接相互作用来抑制φX174 DNA链延伸过程。
