Abbott V, Deloria L, Guenthner T, Jeffery E, Kotake A, Nerland D, Mannering G
Drug Metab Dispos. 1976 May-Jun;4(3):215-22.
Hepatic microsomes from rats fed a crude or a purified diet were compared by measureing their contents of protein, cytochrome P-450, and cytochrome b5, their rates of activity of NADPH- and NADH-cytochrome c reductases, NADPH-cytochrome P-450 reductase, NADPH oxidase, lipid peroxidase, ethylmorphine N-demethylase, aniline hydroxylase, benzpyrene hydroxylase, and their substrate-binding spectra (ethylmorphine, hexobarbital, aniline, and ethyl isoyanide). With the exception of lipid peroxidase activity, which was much higher in microsomes from animals fed the crude diet, little or no consistent diet-related differences in these measurements were observed over a 4-week experimental period, nor were results significantly less variable with one or the other diet. No consistent significant differences were observed with two strains of rats. The lower lipid peroxidase activity seen with the purified diet appeared to be due to the high vitamin E intake when that diet was employed; rats fed the crude diet and an oral supplement of alpha-tocopherol yielded microsomes with low lipid peroxidase activities similar to those seen in microsomes from rats fed the purified diet. A gradual temporal increase in benzpyrene hydroxylase activity was observed with both diets. This was interpreted to be due to environment inducing agents other than those present in the diet.
通过测量喂食粗饲料或纯化饲料的大鼠肝微粒体中的蛋白质、细胞色素P-450和细胞色素b5含量、NADPH-和NADH-细胞色素c还原酶、NADPH-细胞色素P-450还原酶、NADPH氧化酶、脂质过氧化物酶、乙基吗啡N-脱甲基酶、苯胺羟化酶、苯并芘羟化酶的活性以及它们的底物结合光谱（乙基吗啡、己巴比妥、苯胺和乙基异氰化物），对这些肝微粒体进行了比较。除了喂食粗饲料的动物的微粒体中脂质过氧化物酶活性高得多外，在4周的实验期内，这些测量中几乎没有观察到与饮食相关的一致差异，而且使用这两种饮食时结果的变异性也没有显著降低。在两种大鼠品系中未观察到一致的显著差异。使用纯化饲料时脂质过氧化物酶活性较低似乎是由于该饲料中维生素E摄入量高；喂食粗饲料并口服α-生育酚补充剂的大鼠产生的微粒体脂质过氧化物酶活性较低，类似于喂食纯化饲料的大鼠微粒体中的活性。两种饮食都观察到苯并芘羟化酶活性随时间逐渐增加。这被解释为是由于饮食中不存在的环境诱导剂所致。
