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用极谱法测量氧分压,95%响应时间为7 - 10毫秒(作者译)

[Polarographic measurements of PO2 with a 95% response time of 7-10 msec (author's transl)].

作者信息

Diether K, Barnikol W K, Döhring W

出版信息

Respiration. 1978;35(1):8-14. doi: 10.1159/000193853.

Abstract

A fast PO2 tracing with 95% delay times of 15-20 msec in routine is necessary for measurement of the PO2 jumps in the expired air, particularly for the precise measurement of the so-called dead-air plateau. A Clark type PO2 electrode has been developed which has the demanded property. This electrode has four characteristic features: (1) a glasscoated Pt cathode with a tip diameter of 0.3 mm (diameter of the Pt wire = 0.1 mm); (2) a differential screwdrive for feeding the cathode 50 micrometer per turn; (3) a 0.2- to 0.3-micrometer-thin polytetrafluorethylene membrane, and (4) a special polishment of the surface of the cathode. The electrode has a 95% response time of 15-20 msec in routine when tracing PO2 changes from 150 to 750 mm Hg. About 30% of the prepared electrodes have response times of 7-10 msec. The output of the electrode in air is stable within the first 3 h (deviation smaller than 0.2%) and then decreases within 10 h approximately 1% per hour. The response times are constant for more than 48 h. The output of the electrode is linear from 0 to 760 mm Hg PO2. Within the accuracy of measurement the output is independent of PCO2.

摘要

为了测量呼出气体中的氧分压跃变,特别是为了精确测量所谓的无效腔平台,常规情况下需要一个95%延迟时间为15 - 20毫秒的快速氧分压描记图。已经开发出一种具有所需特性的克拉克型氧分压电极。该电极具有四个特征:(1)一个玻璃涂层的铂阴极,尖端直径为0.3毫米(铂丝直径 = 0.1毫米);(2)一个差动螺丝刀,用于使阴极每转动一圈进给50微米;(3)一个0.2至0.3微米厚的聚四氟乙烯膜,以及(4)阴极表面的特殊抛光处理。当追踪氧分压从150毫米汞柱变化到750毫米汞柱时,该电极在常规情况下的95%响应时间为15 - 20毫秒。大约30%制备好的电极响应时间为7 - 10毫秒。该电极在空气中的输出在前3小时内稳定(偏差小于0.2%),然后在10小时内以每小时约1%的速率下降。响应时间在超过48小时内保持恒定。该电极的输出在0至760毫米汞柱氧分压范围内呈线性。在测量精度范围内,输出与二氧化碳分压无关。

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