Derrick K S
Science. 1978 Feb 3;199(4328):538-9. doi: 10.1126/science.622553.
Serologically specific electron microscopy was used to detect double-stranded RNA in extracts of tobacco infected with tobacco mosaic virus. Assays were made immediately after extraction, without purification, concentration, or treatment with phenol or detergent. This indicates that the double-stranded RNA is native and is not an artifact induced by purification methods.
采用血清学特异性电子显微镜技术检测感染烟草花叶病毒的烟草提取物中的双链RNA。提取后立即进行检测,无需纯化、浓缩或用苯酚或去污剂处理。这表明双链RNA是天然存在的,而非纯化方法诱导产生的假象。